Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 8987..9660 Replicon plasmid pHelC
Accession NZ_CP092921
Organism Pseudomonas syringae pv. helianthi strain LMG 5067

Toxin (Protein)


Gene name vapC Uniprot ID A0A2V0QPW6
Locus tag MME54_RS28300 Protein ID WP_017684243.1
Coordinates 9241..9660 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag MME54_RS28295 Protein ID WP_003381346.1
Coordinates 8987..9244 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MME54_RS28265 (MME54_28265) 4101..5015 + 915 WP_054071525.1 DUF4868 domain-containing protein -
MME54_RS28270 (MME54_28270) 5076..6005 - 930 WP_054987848.1 DUF4928 family protein -
MME54_RS28275 (MME54_28275) 6005..7153 - 1149 WP_054987849.1 DNA cytosine methyltransferase -
MME54_RS28280 (MME54_28280) 7580..7942 + 363 WP_007247606.1 hypothetical protein -
MME54_RS28285 (MME54_28285) 7945..8172 + 228 WP_004642455.1 hypothetical protein -
MME54_RS28290 (MME54_28290) 8248..8880 - 633 WP_054987853.1 recombinase family protein -
MME54_RS28295 (MME54_28295) 8987..9244 + 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
MME54_RS28300 (MME54_28300) 9241..9660 + 420 WP_017684243.1 type II toxin-antitoxin system VapC family toxin Toxin
MME54_RS28305 (MME54_28305) 9701..10310 + 610 Protein_12 recombinase family protein -
MME54_RS28310 (MME54_28310) 10609..11544 + 936 WP_054987850.1 AvrD family protein -
MME54_RS28315 (MME54_28315) 11885..12532 + 648 WP_005746177.1 histidine phosphatase family protein -
MME54_RS28320 (MME54_28320) 12771..13598 + 828 WP_054987851.1 TauD/TfdA family dioxygenase -
MME54_RS28325 (MME54_28325) 13616..14650 + 1035 WP_054987854.1 sulfotransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..51306 51306


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15152.46 Da        Isoelectric Point: 4.7010

>T237417 WP_017684243.1 NZ_CP092921:9241-9660 [Pseudomonas syringae pv. helianthi]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAIETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Download         Length: 420 bp

>T237417 NZ_LR882052:2576748-2576855 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT237417 WP_003381346.1 NZ_CP092921:8987-9244 [Pseudomonas syringae pv. helianthi]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp

>AT237417 NZ_LR882052:c2576701-2576635 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPW6

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References