Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 56230..56804 Replicon plasmid pHelB
Accession NZ_CP092920
Organism Pseudomonas syringae pv. helianthi strain LMG 5067

Toxin (Protein)


Gene name vapC Uniprot ID Q48BC5
Locus tag MME54_RS28205 Protein ID WP_003344395.1
Coordinates 56230..56607 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48BC4
Locus tag MME54_RS28210 Protein ID WP_004644067.1
Coordinates 56604..56804 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MME54_RS28185 (MME54_28185) 51446..52024 + 579 WP_053486700.1 conjugal transfer protein TraX -
MME54_RS28190 (MME54_28190) 52155..54299 + 2145 WP_054984026.1 DotA/TraY family protein -
MME54_RS28195 (MME54_28195) 54286..54945 + 660 WP_054984036.1 plasmid IncI1-type surface exclusion protein ExcA -
MME54_RS28200 (MME54_28200) 55038..56240 + 1203 WP_054984027.1 hypothetical protein -
MME54_RS28205 (MME54_28205) 56230..56607 - 378 WP_003344395.1 PIN domain-containing protein Toxin
MME54_RS28210 (MME54_28210) 56604..56804 - 201 WP_004644067.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MME54_RS28215 (MME54_28215) 56897..57982 + 1086 WP_054984028.1 thioredoxin fold domain-containing protein -
MME54_RS28220 (MME54_28220) 57969..60257 + 2289 WP_054984029.1 F-type conjugative transfer protein TrbC -
MME54_RS28225 (MME54_28225) 60390..60707 + 318 WP_241409888.1 hypothetical protein -
MME54_RS28230 (MME54_28230) 60728..61291 + 564 WP_054984031.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..62612 62612


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-43)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13633.73 Da        Isoelectric Point: 6.4723

>T237416 WP_003344395.1 NZ_CP092920:c56607-56230 [Pseudomonas syringae pv. helianthi]
VKGVLVDTSVWVEHFRNNSPELVNLLSQDRVLIHPMVIGELACGTPPDRSNTLTDLGDLRGAQQPTVSEVIAFLNTHKLY
GLGCGLVDMTLLASALLSGTALWTLDKRLERLASRMAVSYQPPTH

Download         Length: 378 bp

>T237416 NZ_LR882052:c2363608-2363432 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7445.66 Da        Isoelectric Point: 7.0102

>AT237416 WP_004644067.1 NZ_CP092920:c56804-56604 [Pseudomonas syringae pv. helianthi]
MRTTVTIDDALYQRALEVADPAMDKADLFREAVQTFVRIQAAKRLMALGATLPTMEDIARRHEKAL

Download         Length: 201 bp

>AT237416 NZ_LR882052:c2363386-2362970 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3GPE2

Antitoxin

Source ID Structure
AlphaFold DB A0A2S4IKB9

References