237415

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 639679..641408 Replicon chromosome
Accession NZ_CP092918
Organism Pseudomonas syringae pv. helianthi strain LMG 5067

Toxin (Protein)


Gene name pmenT Uniprot ID A0A0N8T227
Locus tag MME54_RS02770 Protein ID WP_054987015.1
Coordinates 640764..641408 (-) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A0P9R7I4
Locus tag MME54_RS02765 Protein ID WP_054987014.1
Coordinates 639679..640767 (-) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MME54_RS02735 (MME54_02735) 634830..635150 + 321 WP_054987009.1 hypothetical protein -
MME54_RS02740 (MME54_02740) 635150..636925 + 1776 WP_054987010.1 hypothetical protein -
MME54_RS02745 (MME54_02745) 636922..637527 + 606 WP_054987011.1 hypothetical protein -
MME54_RS02750 (MME54_02750) 637582..638625 + 1044 WP_054987012.1 WavE lipopolysaccharide synthesis family protein -
MME54_RS02755 (MME54_02755) 638656..638901 - 246 WP_054987013.1 helix-turn-helix transcriptional regulator -
MME54_RS02760 (MME54_02760) 639023..639385 + 363 WP_241409747.1 hypothetical protein -
MME54_RS02765 (MME54_02765) 639679..640767 - 1089 WP_054987014.1 macro domain-containing protein Antitoxin
MME54_RS02770 (MME54_02770) 640764..641408 - 645 WP_054987015.1 DUF4433 domain-containing protein Toxin
MME54_RS02775 (MME54_02775) 641405..641986 - 582 WP_054987016.1 DarT ssDNA thymidine ADP-ribosyltransferase family protein -
MME54_RS02780 (MME54_02780) 642985..643923 + 939 WP_054987025.1 DUF4007 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 639679..678713 39034


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24634.19 Da        Isoelectric Point: 7.5091

>T237415 WP_054987015.1 NZ_CP092918:c641408-640764 [Pseudomonas syringae pv. helianthi]
MKYSSLNPKKALIWRIVHRDNLPWILDNGLHCASSDVQAPQYVNIGNLDLIGKRRNRGVPIAPAGVLSDYVPFYFTPFSV
MMKNIHSGRSVQQRSNDEIVILVSSLPRVEQLGLAFVFTNAHAYPDWTDYYSELASLDQIDWSIIQRRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYDDAMKERIEQDVAARGLTLPVHARPGWYFQ

Download         Length: 645 bp

>T237415 NZ_LR882052:2278017-2279339 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40454.46 Da        Isoelectric Point: 6.5081

>AT237415 WP_054987014.1 NZ_CP092918:c640767-639679 [Pseudomonas syringae pv. helianthi]
MIRFTQGNLLKADTEALVNTVNTVGVMGKGIALMFKERFTENYRLYSAACKAGEVETGKVHVTAVNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLRDLRRFLIDNQVKSVAVPPLGAGNGGLKWVEVREQIVDVLSDLDVDVLVFEPTDQYLNVAKHSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIEQLPSAKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGINDVIWFDEGRKAFLQTYLKTEAKEYSQALERTAELIDGFESPFGMELLATVDWLLSKSGVSPTVPAVREA
LKNWDGGAGAAARKSRLFDDQALGIALKRLTASNLSPEIAVP

Download         Length: 1089 bp

>AT237415 NZ_LR882052:2277751-2278017 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0N8T227

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9R7I4

References