Detailed information of TA system TA237391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 448154..448730
• Replicon: chromosome
• Accession: NZ_CP092911
• Organism: Salmonella enterica subsp. enterica strain SR33

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: MLD58_RS02110
• Protein ID: WP_001131963.1
• Coordinates: 448154..448441 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A6C6ZAR7
• Locus tag: MLD58_RS02115
• Protein ID: WP_000063143.1
• Coordinates: 448428..448730 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLD58_RS02100 (443264)	443264..446773	-	3510	WP_023138516.1	type I restriction-modification system endonuclease	-
MLD58_RS02105 (446971)	446971..447885	+	915	WP_023138517.1	restriction endonuclease	-
MLD58_RS02110 (448154)	448154..448441	+	288	WP_001131963.1	BrnT family toxin	Toxin
MLD58_RS02115 (448428)	448428..448730	+	303	WP_000063143.1	BrnA antitoxin family protein	Antitoxin
MLD58_RS02120 (448805)	448805..449761	-	957	WP_000187840.1	GTPase	-
MLD58_RS02125 (449772)	449772..449975	-	204	WP_000460616.1	YbdD/YjiX family protein	-
MLD58_RS02130 (450070)	450070..452220	-	2151	WP_000379928.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (40-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T237391 WP_001131963.1 NZ_CP092911:448154-448441 [Salmonella enterica subsp. enterica]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T237391 NZ_LR882050:c2077922-2077524 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11402.01 Da
• Isoelectric Point: 10.1293

>AT237391 WP_000063143.1 NZ_CP092911:448428-448730 [Salmonella enterica subsp. enterica]
MSMVKHKRGNASALSAQHEAELKALVKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT237391 NZ_LR882050:c2078218-2077925 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A6C6ZAR7