Detailed information of TA system TA237352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 6026..6936
• Replicon: plasmid pA_F11
• Accession: NZ_CP092902
• Organism: Klebsiella pneumoniae strain F11

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: F11wdg_RS25730
• Protein ID: WP_004026354.1
• Coordinates: 6466..6936 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: F11wdg_RS25725
• Protein ID: WP_004181895.1
• Coordinates: 6026..6469 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F11wdg_RS25710 (F11wdg_pA0003)	2659..4146	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
F11wdg_RS25715 (F11wdg_pA0004)	4232..4654	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
F11wdg_RS25720 (F11wdg_pA0005)	4654..5925	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
F11wdg_RS25725 (F11wdg_pA0006)	6026..6469	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
F11wdg_RS25730 (F11wdg_pA0007)	6466..6936	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
F11wdg_RS25735 (F11wdg_pA0008)	7046..7306	-	261	WP_004026352.1	hypothetical protein	-
F11wdg_RS25740	8005..9373	+	1369	Protein_7	IS3-like element ISKpn8 family transposase	-
F11wdg_RS25745 (F11wdg_pA0012)	9370..10503	-	1134	WP_172694432.1	IS481-like element ISKpn28 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-14 / qacE / catB3 / blaOXA-1 / fosE	-	1..221757	221757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T237352 WP_004026354.1 NZ_CP092902:6466-6936 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T237352 NZ_LR881938:c4517547-4517290 [Escherichia coli str. K-12 substr. MG1655]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT237352 WP_004181895.1 NZ_CP092902:6026-6469 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT237352 NZ_LR881938:c4517278-4516733 [Escherichia coli str. K-12 substr. MG1655]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A2J4RLY9