237351

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5208414..5209039 Replicon chromosome
Accession NZ_CP092901
Organism Klebsiella pneumoniae strain F11

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag F11wdg_RS25340 Protein ID WP_019705794.1
Coordinates 5208414..5208797 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag F11wdg_RS25345 Protein ID WP_004150355.1
Coordinates 5208797..5209039 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F11wdg_RS25325 (5205780) 5205780..5206682 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
F11wdg_RS25330 (5206679) 5206679..5207314 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
F11wdg_RS25335 (5207311) 5207311..5208240 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
F11wdg_RS25340 (5208414) 5208414..5208797 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
F11wdg_RS25345 (5208797) 5208797..5209039 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
F11wdg_RS25350 (5209244) 5209244..5210161 + 918 WP_004146235.1 alpha/beta hydrolase -
F11wdg_RS25355 (5210176) 5210176..5211117 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
F11wdg_RS25360 (5211162) 5211162..5211599 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
F11wdg_RS25365 (5211596) 5211596..5212456 - 861 WP_241097818.1 virulence factor BrkB family protein -
F11wdg_RS25370 (5212450) 5212450..5213049 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T237351 WP_019705794.1 NZ_CP092901:c5208797-5208414 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T237351 NZ_LR881938:4432186-4432536 [Escherichia coli str. K-12 substr. MG1655]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT237351 WP_004150355.1 NZ_CP092901:c5209039-5208797 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT237351 NZ_LR881938:4431941-4432192 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References