23735

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 450016..450782 Replicon chromosome II
Accession NC_012583
Organism Vibrio cholerae O395

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag VC395_RS16600 Protein ID WP_000982260.1
Coordinates 450016..450513 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag VC395_RS16605 Protein ID WP_000246253.1
Coordinates 450510..450782 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VC395_RS16565 445677..445952 + 276 WP_000095573.1 hypothetical protein -
VC395_RS16570 445969..446346 + 378 WP_000073388.1 hypothetical protein -
VC395_RS16575 446361..446762 + 402 WP_012706059.1 hypothetical protein -
VC395_RS16585 447249..448907 - 1659 WP_001125478.1 recombinase family protein -
VC395_RS16595 449225..449719 - 495 WP_000477435.1 hypothetical protein -
VC395_RS16600 450016..450513 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
VC395_RS16605 450510..450782 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
VC395_RS16610 451013..451681 + 669 WP_000043871.1 hypothetical protein -
VC395_RS16615 451833..452120 + 288 WP_000426470.1 hypothetical protein -
VC395_RS16620 452261..452632 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
VC395_RS21085 452699..453124 + 426 WP_001882332.1 DUF1778 domain-containing protein -
VC395_RS16630 453121..453654 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
VC395_RS16635 453856..454113 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VC395_RS16640 454101..454403 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
VC395_RS16645 454637..455554 + 918 WP_000186333.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 427343..471178 43835
- inside Integron - - 450510..464084 13574


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T23735 WP_000982260.1 NC_012583:c450513-450016 [Vibrio cholerae O395]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T23735 NC_012583:c450513-450016 [Vibrio cholerae O395]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT23735 WP_000246253.1 NC_012583:c450782-450510 [Vibrio cholerae O395]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT23735 NC_012583:c450782-450510 [Vibrio cholerae O395]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References