Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3966637..3967256 Replicon chromosome
Accession NZ_CP092901
Organism Klebsiella pneumoniae strain F11

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag F11wdg_RS19500 Protein ID WP_002892050.1
Coordinates 3967038..3967256 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag F11wdg_RS19495 Protein ID WP_002892066.1
Coordinates 3966637..3967011 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F11wdg_RS19485 (3961789) 3961789..3962982 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
F11wdg_RS19490 (3963005) 3963005..3966151 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
F11wdg_RS19495 (3966637) 3966637..3967011 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
F11wdg_RS19500 (3967038) 3967038..3967256 + 219 WP_002892050.1 HHA domain-containing protein Toxin
F11wdg_RS19505 (3967419) 3967419..3967985 + 567 WP_002892030.1 maltose O-acetyltransferase -
F11wdg_RS19510 (3967957) 3967957..3968097 - 141 WP_004147370.1 hypothetical protein -
F11wdg_RS19515 (3968118) 3968118..3968588 + 471 WP_002892026.1 YlaC family protein -
F11wdg_RS19520 (3968563) 3968563..3970014 - 1452 WP_064151794.1 PLP-dependent aminotransferase family protein -
F11wdg_RS19525 (3970115) 3970115..3970813 + 699 WP_002892021.1 GNAT family protein -
F11wdg_RS19530 (3970810) 3970810..3970950 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
F11wdg_RS19535 (3970950) 3970950..3971213 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T237347 WP_002892050.1 NZ_CP092901:3967038-3967256 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T237347 NZ_LR881938:c3217946-3217632 [Escherichia coli str. K-12 substr. MG1655]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT237347 WP_002892066.1 NZ_CP092901:3966637-3967011 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT237347 NZ_LR881938:c3217635-3217219 [Escherichia coli str. K-12 substr. MG1655]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References