Detailed information of TA system TA237334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 170875..171473
• Replicon: plasmid pLN1-183666
• Accession: NZ_CP092895
• Organism: Agrobacterium tumefaciens strain LN-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MLE07_RS26445
• Protein ID: WP_078053756.1
• Coordinates: 170875..171168 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MLE07_RS26450
• Protein ID: WP_078053757.1
• Coordinates: 171165..171473 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLE07_RS26435 (MLE07_26435)	166441..166683	-	243	WP_078053834.1	hypothetical protein	-
MLE07_RS26440 (MLE07_26440)	167496..170603	+	3108	WP_236762456.1	autotransporter outer membrane beta-barrel domain-containing protein	-
MLE07_RS26445 (MLE07_26445)	170875..171168	-	294	WP_078053756.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
MLE07_RS26450 (MLE07_26450)	171165..171473	-	309	WP_078053757.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MLE07_RS26455 (MLE07_26455)	171756..172241	-	486	WP_114836293.1	hypothetical protein	-
MLE07_RS26460 (MLE07_26460)	172394..172513	+	120	Protein_179	nitroreductase family protein	-
MLE07_RS26465 (MLE07_26465)	173175..173681	+	507	WP_112967606.1	IS3 family transposase	-
MLE07_RS26470 (MLE07_26470)	173911..174210	-	300	WP_078053759.1	DUF982 domain-containing protein	-
MLE07_RS26475 (MLE07_26475)	174288..174761	-	474	WP_078053760.1	Hsp20 family protein	-
MLE07_RS26480 (MLE07_26480)	175269..175904	-	636	WP_223565061.1	IS630 family transposase	-
MLE07_RS26485 (MLE07_26485)	175829..176212	-	384	WP_223565060.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..183666	183666
	-	flank	IS/Tn	-	-	175269..175856	587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-59)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10746.27 Da
• Isoelectric Point: 5.7995

>T237334 WP_078053756.1 NZ_CP092895:c171168-170875 [Agrobacterium tumefaciens]
VKLTWSAFALSDRDAIFTYIEADNPAAAILIDERIVSAARRLLDFPASGRLGRIAGTRELVINGTPYVAAYATTETTVRI
LRVLHGAQEWPEYLLQN

Nucleotide

• Length: 294 bp

>T237334 NZ_LR881938:c1398196-1398002 [Escherichia coli str. K-12 substr. MG1655]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11084.42 Da
• Isoelectric Point: 4.8892

>AT237334 WP_078053757.1 NZ_CP092895:c171473-171165 [Agrobacterium tumefaciens]
MAANALVQTRIDAEVRDRASAVLESMGLTVSDAVRIMLTRTANEGALPLELLSGSETHDAWFRTKVLEALNDTRSDLTDD
EAEAHFAKRRAAARLKVGSTES

Nucleotide

• Length: 309 bp

>AT237334 NZ_LR881938:1397825-1398003 [Escherichia coli str. K-12 substr. MG1655]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT