Detailed information of TA system TA237333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 2246156..2246744
• Replicon: chromosome
• Accession: NZ_CP092893
• Organism: Agrobacterium tumefaciens strain LN-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: MLE07_RS24375
• Protein ID: WP_243017536.1
• Coordinates: 2246442..2246744 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: MLE07_RS24370
• Protein ID: WP_243017501.1
• Coordinates: 2246156..2246449 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLE07_RS24335 (MLE07_24335)	2241422..2241805	-	384	WP_243017494.1	ester cyclase	-
MLE07_RS24340 (MLE07_24340)	2241899..2242483	-	585	WP_243017495.1	histidine phosphatase family protein	-
MLE07_RS24345 (MLE07_24345)	2242507..2243067	-	561	WP_243017496.1	AAA family ATPase	-
MLE07_RS26580	2243185..2243313	-	129	WP_279238304.1	hypothetical protein	-
MLE07_RS24350 (MLE07_24350)	2243436..2243756	-	321	WP_243017497.1	hypothetical protein	-
MLE07_RS24355 (MLE07_24355)	2244164..2244697	+	534	WP_243017498.1	hypothetical protein	-
MLE07_RS24360 (MLE07_24360)	2244880..2245296	-	417	WP_243017499.1	hypothetical protein	-
MLE07_RS24365 (MLE07_24365)	2245295..2245927	+	633	WP_243017500.1	CatB-related O-acetyltransferase	-
MLE07_RS24370 (MLE07_24370)	2246156..2246449	-	294	WP_243017501.1	putative addiction module antidote protein	Antitoxin
MLE07_RS24375 (MLE07_24375)	2246442..2246744	-	303	WP_243017536.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MLE07_RS24380 (MLE07_24380)	2246957..2247787	+	831	WP_243017502.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
MLE07_RS24385 (MLE07_24385)	2247774..2248694	+	921	WP_243017503.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MLE07_RS24390 (MLE07_24390)	2248721..2249089	-	369	WP_243017504.1	transcriptional regulator	-
MLE07_RS24395 (MLE07_24395)	2249086..2249376	-	291	WP_243017505.1	DUF6516 family protein	-
MLE07_RS24400 (MLE07_24400)	2250377..2250667	+	291	WP_243017505.1	DUF6516 family protein	-
MLE07_RS24405 (MLE07_24405)	2250664..2251032	+	369	WP_243017504.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2229483..2260346	30863

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11232.93 Da
• Isoelectric Point: 9.9209

>T237333 WP_243017536.1 NZ_CP092893:c2246744-2246442 [Agrobacterium tumefaciens]
IELKQTAVFAKWETRLRDKRARTIIAARLMRLAEGLPGDVEPVGEGVSELRIHYGPGYRVYFQRRGNVLIVLLCGGDKGS
QARDIAAAHKLAKEWSEKDD

Nucleotide

• Length: 303 bp

>T237333 NZ_LR881938:c1269569-1269462 [Escherichia coli str. K-12 substr. MG1655]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10383.81 Da
• Isoelectric Point: 5.8373

>AT237333 WP_243017501.1 NZ_CP092893:c2246449-2246156 [Agrobacterium tumefaciens]
MTEKLTTYDPAAALVDDEEIAAFMADAFETGDAAYVAKALGVVARAKGMTEISRKTGLSREQLYRSFSERGNPTLKTTLA
VMRALGVDVTAKAHTAQ

Nucleotide

• Length: 294 bp

>AT237333 NZ_LR881938:1269616-1269682 [Escherichia coli str. K-12 substr. MG1655]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT