Detailed information of TA system TA237330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 451905..452449
• Replicon: chromosome
• Accession: NZ_CP092892
• Organism: Agrobacterium tumefaciens strain LN-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: MLE07_RS02215
• Protein ID: WP_026363704.1
• Coordinates: 452159..452449 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A822V4A8
• Locus tag: MLE07_RS02210
• Protein ID: WP_003509084.1
• Coordinates: 451905..452156 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLE07_RS02185 (MLE07_02185)	447666..448346	+	681	WP_003509096.1	GntR family transcriptional regulator	-
MLE07_RS02190 (MLE07_02190)	448392..448868	+	477	WP_049832327.1	DUF1284 domain-containing protein	-
MLE07_RS02195 (MLE07_02195)	449044..450144	-	1101	WP_243016335.1	hypothetical protein	-
MLE07_RS02200 (MLE07_02200)	450110..450322	+	213	WP_243016337.1	hypothetical protein	-
MLE07_RS02205 (MLE07_02205)	450381..451526	+	1146	WP_087743499.1	site-specific DNA-methyltransferase	-
MLE07_RS02210 (MLE07_02210)	451905..452156	+	252	WP_003509084.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
MLE07_RS02215 (MLE07_02215)	452159..452449	+	291	WP_026363704.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MLE07_RS02220 (MLE07_02220)	452491..453102	-	612	WP_087743505.1	HAD family phosphatase	-
MLE07_RS02225 (MLE07_02225)	453099..454211	-	1113	WP_111793355.1	A/G-specific adenine glycosylase	-
MLE07_RS02230 (MLE07_02230)	454339..454803	+	465	WP_003509080.1	hypothetical protein	-
MLE07_RS02235 (MLE07_02235)	454831..455352	+	522	WP_243016339.1	DUF721 domain-containing protein	-
MLE07_RS02240 (MLE07_02240)	455481..456161	+	681	WP_003509078.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (12-70)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11195.86 Da
• Isoelectric Point: 6.2182

>T237330 WP_026363704.1 NZ_CP092892:452159-452449 [Agrobacterium tumefaciens]
VGFRLSLLAEEDVIRIAEEGIAIFGLAQAQKYHRELYEIFELISLNPRMARERHELLPPLRIHRFRAHLVVYGVGADDDV
LIVRVRHAHEDWVNES

Nucleotide

• Length: 291 bp

>T237330 NZ_LR881938:c479532-479314 [Escherichia coli str. K-12 substr. MG1655]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9339.55 Da
• Isoelectric Point: 9.7317

>AT237330 WP_003509084.1 NZ_CP092892:451905-452156 [Agrobacterium tumefaciens]
MATMNISLPAPMKEWVEAQSRTGRYSNASDYVRDLIRKDQERGEKIAAMQHFVDEGLQSGAGHRSQQALFAEAEARSMKR
KIG

Nucleotide

• Length: 252 bp

>AT237330 NZ_LR881938:c479932-479558 [Escherichia coli str. K-12 substr. MG1655]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A822V4A8