Detailed information of TA system TA237329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 330626..331106
• Replicon: chromosome
• Accession: NZ_CP092892
• Organism: Agrobacterium tumefaciens strain LN-1

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: MLE07_RS01600
• Protein ID: WP_111781272.1
• Coordinates: 330626..330895 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: F0L6V5
• Locus tag: MLE07_RS01605
• Protein ID: WP_013635548.1
• Coordinates: 330879..331106 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLE07_RS01565 (MLE07_01565)	325689..326318	+	630	WP_020010519.1	DNA-3-methyladenine glycosylase I	-
MLE07_RS01570 (MLE07_01570)	326315..326761	+	447	WP_080865875.1	hypothetical protein	-
MLE07_RS01575 (MLE07_01575)	326777..327820	-	1044	WP_065694890.1	YeiH family protein	-
MLE07_RS01580 (MLE07_01580)	327902..328819	+	918	WP_026363679.1	LysR substrate-binding domain-containing protein	-
MLE07_RS01585 (MLE07_01585)	328990..329363	-	374	Protein_310	DUF305 domain-containing protein	-
MLE07_RS01590 (MLE07_01590)	329421..329813	-	393	WP_243016293.1	hypothetical protein	-
MLE07_RS01595 (MLE07_01595)	329855..330619	-	765	WP_243016295.1	hypothetical protein	-
MLE07_RS01600 (MLE07_01600)	330626..330895	-	270	WP_111781272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MLE07_RS01605 (MLE07_01605)	330879..331106	-	228	WP_013635548.1	DUF6290 family protein	Antitoxin
MLE07_RS01610 (MLE07_01610)	331264..332787	+	1524	WP_243016297.1	histidine--tRNA ligase	-
MLE07_RS01615 (MLE07_01615)	332792..333160	+	369	WP_099085622.1	VOC family protein	-
MLE07_RS01620 (MLE07_01620)	333177..334304	+	1128	WP_243016299.1	ATP phosphoribosyltransferase regulatory subunit	-
MLE07_RS01625 (MLE07_01625)	334301..334993	+	693	WP_003503184.1	ATP phosphoribosyltransferase	-
MLE07_RS01630 (MLE07_01630)	335056..335502	-	447	WP_003503183.1	DoxX family protein	-
MLE07_RS01635 (MLE07_01635)	335659..336093	-	435	WP_003503182.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10715.36 Da
• Isoelectric Point: 9.9415

>T237329 WP_111781272.1 NZ_CP092892:c330895-330626 [Agrobacterium tumefaciens]
VIWTIEYHTLVQKEMRKINPETRRRIRSFLHERLAALDDPRQTGAALQGSELGNFWRYRVGDYRIICDIQDHKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T237329 NZ_LR881938:c262893-262552 [Escherichia coli str. K-12 substr. MG1655]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8909.92 Da
• Isoelectric Point: 4.7254

>AT237329 WP_013635548.1 NZ_CP092892:c331106-330879 [Agrobacterium tumefaciens]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRIQRGESKVVSAEEFWRDLDN

Nucleotide

• Length: 228 bp

>AT237329 NZ_LR881938:c263231-262914 [Escherichia coli str. K-12 substr. MG1655]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Antitoxin

• AlphaFold DB: F0L6V5