Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1592612..1593161 Replicon chromosome
Accession NZ_CP092888
Organism Bifidobacterium sp. FKU

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag MMB48_RS07300 Protein ID WP_003829109.1
Coordinates 1592838..1593161 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0M4LW25
Locus tag MMB48_RS07295 Protein ID WP_032743660.1
Coordinates 1592612..1592851 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMB48_RS07275 (MMB48_07230) 1588456..1589118 + 663 WP_032743761.1 TetR/AcrR family transcriptional regulator -
MMB48_RS07280 (MMB48_07235) 1589275..1590546 + 1272 WP_032743663.1 MFS transporter -
MMB48_RS07285 (MMB48_07240) 1590730..1590840 - 111 WP_230581173.1 XRE family transcriptional regulator -
MMB48_RS07290 (MMB48_07245) 1591176..1592432 - 1257 WP_032743661.1 HipA domain-containing protein -
MMB48_RS07295 (MMB48_07250) 1592612..1592851 + 240 WP_032743660.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
MMB48_RS07300 (MMB48_07255) 1592838..1593161 + 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MMB48_RS07305 (MMB48_07260) 1593378..1593992 - 615 WP_080714683.1 cupin domain-containing protein -
MMB48_RS07310 (MMB48_07265) 1594140..1594499 - 360 WP_080714682.1 class I SAM-dependent methyltransferase -
MMB48_RS07315 (MMB48_07270) 1594737..1595102 + 366 WP_032743659.1 YccF domain-containing protein -
MMB48_RS07320 (MMB48_07275) 1595644..1596909 + 1266 WP_050496214.1 Fic family protein -
MMB48_RS07325 (MMB48_07280) 1597379..1597668 + 290 Protein_1422 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-54)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T237326 WP_003829109.1 NZ_CP092888:1592838-1593161 [Bifidobacterium sp. FKU]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T237326 NZ_LR881937:c87299-86817 [Enterobacter cancerogenus]
GTGGGACGTTTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTGGCTGAGTTCGTCAGCGGTGAGGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCCCTCGGAGCTGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGCGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAGCGAACATTGTTTCTTAGACTCCCT
TAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9121.16 Da        Isoelectric Point: 4.5881

>AT237326 WP_032743660.1 NZ_CP092888:1592612-1592851 [Bifidobacterium sp. FKU]
MQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDETR

Download         Length: 240 bp

>AT237326 NZ_LR881937:c87574-87287 [Enterobacter cancerogenus]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGCGGAGTTCATCGACATGCTTGATGCACCTGTCGTAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M4LW25

References