Detailed information of TA system TA237325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 1533519..1534158
• Replicon: chromosome
• Accession: NZ_CP092888
• Organism: Bifidobacterium sp. FKU

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W6EZW1
• Locus tag: MMB48_RS07010
• Protein ID: WP_014484977.1
• Coordinates: 1533802..1534158 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MMB48_RS07005
• Protein ID: WP_003829235.1
• Coordinates: 1533519..1533815 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMB48_RS06980 (MMB48_06935)	1528910..1531588	-	2679	WP_032743694.1	alanine--tRNA ligase	-
MMB48_RS06985 (MMB48_06940)	1531717..1531953	-	237	WP_007053386.1	hypothetical protein	-
MMB48_RS06990 (MMB48_06945)	1531953..1532351	-	399	WP_032743693.1	DUF948 domain-containing protein	-
MMB48_RS06995 (MMB48_06950)	1532434..1533108	-	675	WP_032743692.1	histidine phosphatase family protein	-
MMB48_RS07000 (MMB48_06955)	1533183..1533449	+	267	WP_230581180.1	hypothetical protein	-
MMB48_RS07005 (MMB48_06960)	1533519..1533815	+	297	WP_003829235.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MMB48_RS07010 (MMB48_06965)	1533802..1534158	+	357	WP_014484977.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MMB48_RS07015 (MMB48_06970)	1534433..1535035	-	603	WP_032743691.1	transglutaminase family protein	-
MMB48_RS07020 (MMB48_06975)	1535140..1535373	-	234	WP_235625961.1	hypothetical protein	-
MMB48_RS07025 (MMB48_06980)	1535390..1535779	+	390	WP_230581178.1	cytidine deaminase	-
MMB48_RS07030 (MMB48_06985)	1535917..1536699	+	783	WP_032743690.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
MMB48_RS07035 (MMB48_06990)	1536878..1537504	-	627	WP_007052130.1	30S ribosomal protein S4	-
MMB48_RS07040 (MMB48_06995)	1537703..1538695	-	993	WP_080714690.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (12-54)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13302.25 Da
• Isoelectric Point: 8.9603

>T237325 WP_014484977.1 NZ_CP092888:1533802-1534158 [Bifidobacterium sp. FKU]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T237325 NZ_LR881936:c3768387-3767977 [Enterobacter cancerogenus]
GTGGTCCTGTGGCAATCTGAACTTCGCGTATCATGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTGGCGGC
CTTTGTACTACTAATGCCGTGGCCCCTCAGCTACACGCCTCTGTGGTTGCTGCTGCTGTCATTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGACGGATTAATGCCCGTCAGGGTGAGATCAAACTGCTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGACATCATCGGTACGCCGTGGATGCTCAATTCCGGCATGATGCTGCGCTTGCGAAGCGTGGATGGAGAGCGTCG
CCAGCACCTGTGGCTGGCGGCTGACAGTATGGACGCTGCCGAGTGGCGGGATCTGCGCCGGACGATGTTGCAGCAGCCGA
CGCAGGATTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10997.50 Da
• Isoelectric Point: 4.2896

>AT237325 WP_003829235.1 NZ_CP092888:1533519-1533815 [Bifidobacterium sp. FKU]
MMAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTP
QIRDGAVILPEGWDDDED

Nucleotide

• Length: 297 bp

>AT237325 NZ_LR881936:c3768634-3768368 [Enterobacter cancerogenus]
ATGGATATTAACAATAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGTATGCGTGAACTTGATATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGACACCCTGAGCGACGACGATAAGCGGCTCTTTGTGCGCCTGCTCGAATCAGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0L7D489