Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 1044837..1045420 Replicon chromosome
Accession NZ_CP092888
Organism Bifidobacterium sp. FKU

Toxin (Protein)


Gene name PumA Uniprot ID A0A1S2VZ23
Locus tag MMB48_RS04615 Protein ID WP_032745284.1
Coordinates 1045118..1045420 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID I3AVV5
Locus tag MMB48_RS04610 Protein ID WP_007057517.1
Coordinates 1044837..1045121 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMB48_RS04585 (MMB48_04570) 1040981..1041349 - 369 WP_155270629.1 hypothetical protein -
MMB48_RS04590 (MMB48_04575) 1041343..1042425 + 1083 Protein_887 transposase -
MMB48_RS04595 (MMB48_04580) 1042523..1043431 + 909 WP_032744304.1 ABC transporter ATP-binding protein -
MMB48_RS04600 (MMB48_04585) 1043428..1044159 + 732 WP_032744305.1 ABC transporter permease -
MMB48_RS04605 (MMB48_04590) 1044177..1044728 - 552 Protein_890 IS30 family transposase -
MMB48_RS04610 (MMB48_04595) 1044837..1045121 - 285 WP_007057517.1 putative addiction module antidote protein Antitoxin
MMB48_RS04615 (MMB48_04600) 1045118..1045420 - 303 WP_032745284.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MMB48_RS04620 (MMB48_04605) 1045458..1045787 - 330 WP_257798116.1 DUF1922 domain-containing protein -
MMB48_RS04625 (MMB48_04610) 1045913..1046401 + 489 WP_032745281.1 D-aminoacyl-tRNA deacylase -
MMB48_RS04630 (MMB48_04615) 1046562..1049681 + 3120 WP_136527129.1 alpha-mannosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1044177..1044632 455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(9-92)

Antitoxin

Uncharacterized protein HI1420

(3-92)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11350.08 Da        Isoelectric Point: 10.4626

>T237323 WP_032745284.1 NZ_CP092888:c1045420-1045118 [Bifidobacterium sp. FKU]
IKQSAEYRKWFKKLRDHKAKAAIQARLDACKLAGRPFGDIKPVGGPVSEMRFHTGAGYRVYFAMQGNVLMLLLAGGDKST
QQTDIRQAHDILNDYKEQRQ

Download         Length: 303 bp

>T237323 NZ_LR881936:1269550-1269936 [Enterobacter cancerogenus]
ATGAGCCACGCGTTAGAATTTATCGAGACGTCACTTTTTACCCGGCAGATCAAAAGTATTGCATCGGATGACGAATTAAA
GGATCTCCAAAAGGAACTCATTGCCTGGCCTGATAAAGGTGACGTGATCCAGCATACCGGAGGCTTGCGTAAAATAAGGA
TGGCCGCTGGGTCAAAGGGAAAGAGAGGGGGAGCCAGAGTCATTTACTTTTTGGCCACGGAAGAGGTGATTTATCTCATC
ATGGCCTATCCAAAAAATACGAAGGACACTTTGACAGAGACAGAGAAAGCACAGCTTAAAAAGCTCACCTCTGTTCTTAG
AAATACTGCTTCCAATGCCCTTGATGAGAGGTGTACTATGTACCCCAATCAGGTAAGAGGAGGTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10158.37 Da        Isoelectric Point: 4.3707

>AT237323 WP_007057517.1 NZ_CP092888:c1045121-1044837 [Bifidobacterium sp. FKU]
MSTEFTDYDTSEYLENEQDIIAYLNAIAEYDDPALMQAALGNVAKARGMTQIAKDAGVGRESLYKSLSKDGNPSFQTIAK
VIHALGGRLTIQAA

Download         Length: 285 bp

>AT237323 NZ_LR881936:1269939-1270223 [Enterobacter cancerogenus]
ATGAGTATTTTTGAAGAACTGAAATCTTCGCTTGAAGAGGCTGTTGAGATCCATAGCGGAAAAAAAACACCCTCCCGAGT
CACTCGCTATGAAGTCGCCGATGTTCGTGCAATCAGAGAACAGCTTAATGTTACGCAAAGCGAAATGGCAAAGGCGCTCG
GCACGAGCGTTGATACCATAAAAAGCTGGGAGTCTAAACGTCGTAACCCAACGGGGCTTGCAGCGAAAGTCCTTAAGGCG
ATTCGGGATAACCCTGCGTTTTACAGCGCGCTTGCTGGGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S2VZ23

Antitoxin

Source ID Structure
AlphaFold DB I3AVV5

References