Detailed information of TA system TA237316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4868223..4868839
• Replicon: chromosome
• Accession: NZ_CP092861
• Organism: Salmonella enterica strain P164045

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8Z2U3
• Locus tag: MLB75_RS24255
• Protein ID: WP_000238494.1
• Coordinates: 4868223..4868597 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8E9YHE9
• Locus tag: MLB75_RS24260
• Protein ID: WP_001523745.1
• Coordinates: 4868597..4868839 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLB75_RS24240 (4865725)	4865725..4866627	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
MLB75_RS24245 (4866624)	4866624..4867259	+	636	WP_241065957.1	formate dehydrogenase cytochrome b556 subunit	-
MLB75_RS24250 (4867256)	4867256..4868185	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
MLB75_RS24255 (4868223)	4868223..4868597	-	375	WP_000238494.1	type II toxin-antitoxin system VapC family toxin	Toxin
MLB75_RS24260 (4868597)	4868597..4868839	-	243	WP_001523745.1	CopG family transcriptional regulator	Antitoxin
MLB75_RS24265 (4869044)	4869044..4869973	+	930	WP_001162860.1	alpha/beta hydrolase	-
MLB75_RS24270 (4870059)	4870059..4870370	+	312	WP_000558163.1	type II toxin-antitoxin system HigB family toxin	-
MLB75_RS24275 (4870367)	4870367..4870813	+	447	WP_001259012.1	type II toxin-antitoxin system HigA family antitoxin	-
MLB75_RS24280 (4870828)	4870828..4871769	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
MLB75_RS24285 (4871814)	4871814..4872251	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
MLB75_RS24290 (4872248)	4872248..4873120	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MLB75_RS24295 (4873114)	4873114..4873713	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13908.18 Da
• Isoelectric Point: 7.3567

>T237316 WP_000238494.1 NZ_CP092861:c4868597-4868223 [Salmonella enterica]
MVKGSALFDTNILIDLFSGRIEAKHALEAYPPQNAISLITWMEVMVGAKKYHQENRTRIALSAFNIIGVTQEIAERSVIV
RQEYGMKLPDAIILATAQVHRCELVTRNTKDFADIPGVITPYHL

Nucleotide

• Length: 375 bp

>T237316 NZ_LR881935:c239174-238887 [Citrobacter freundii]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.77 Da
• Isoelectric Point: 4.4463

>AT237316 WP_001523745.1 NZ_CP092861:c4868839-4868597 [Salmonella enterica]
MMAGMDMGRILLDLSDDVIKRLDDLKVQRNLPRAELLREAVEQYLERQDRAETTISKALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT237316 NZ_LR881935:c239413-239174 [Citrobacter freundii]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A5I6CND8