Detailed information of TA system TA237302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1062373..1063187
• Replicon: chromosome
• Accession: NZ_CP092861
• Organism: Salmonella enterica strain P164045

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: B5RDM7
• Locus tag: MLB75_RS05195
• Protein ID: WP_000971654.1
• Coordinates: 1062373..1062900 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: A0A4Y6M833
• Locus tag: MLB75_RS05200
• Protein ID: WP_072162154.1
• Coordinates: 1062897..1063187 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLB75_RS05165 (1058344)	1058344..1059012	+	669	WP_000445914.1	hypothetical protein	-
MLB75_RS05170 (1059039)	1059039..1059242	+	204	Protein_1016	hypothetical protein	-
MLB75_RS05175 (1059296)	1059296..1060551	+	1256	WP_086016038.1	IS3-like element ISSen1 family transposase	-
MLB75_RS05180 (1060732)	1060732..1060848	+	117	Protein_1018	hypothetical protein	-
MLB75_RS05185 (1061092)	1061092..1061748	-	657	WP_031604883.1	protein-serine/threonine phosphatase	-
MLB75_RS05190 (1062077)	1062077..1062300	+	224	Protein_1020	IS5/IS1182 family transposase	-
MLB75_RS05195 (1062373)	1062373..1062900	-	528	WP_000971654.1	GNAT family N-acetyltransferase	Toxin
MLB75_RS05200 (1062897)	1062897..1063187	-	291	WP_072162154.1	DUF1778 domain-containing protein	Antitoxin
MLB75_RS05205 (1063457)	1063457..1063635	-	179	Protein_1023	IS3 family transposase	-
MLB75_RS05210 (1064039)	1064039..1064482	-	444	WP_000715096.1	SPI-1 type III secretion system invasion lipoprotein InvH	-
MLB75_RS05215 (1064938)	1064938..1065588	+	651	WP_001674874.1	type III secretion system transcriptional activator InvF	-
MLB75_RS05220 (1065585)	1065585..1067273	+	1689	WP_000848114.1	type III secretion system outer membrane ring protein InvG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19041.85 Da
• Isoelectric Point: 9.6420

>T237302 WP_000971654.1 NZ_CP092861:c1062900-1062373 [Salmonella enterica]
MMFTDWHEAAIGKTHNRMNFDCGDADLNQFLQRHARQNHEKGTTKTYVALDNSDVTRIHGFYSVSPASLIYAQVPGAISK
GLGRYDVPVFRLGRLAVDKSMQGQGLGAQLLLSAGKRCIQAALQVGGVALLIDAKNKQACDWYKGFGAVPLNDQPLSLLL
SFKTLYAALSASGRL

Nucleotide

• Length: 528 bp

>T237302 NZ_LR881183:c878261-877860 [Thermococcus camini]
ATGCATGGAGTGATAGATACCAACGTCCTCATCTATGACACATTCGAGGACTCGGAGTTTCACATAGAGGCCGAAAAAGT
TCTGGAGTCCCTCGATACGTGGTATATACCCCCGATAGTCCTTCAGGAGTTCGTCTGGTTCTTCAAGAACAACGGGTTTT
CCGTTGATGAGACGCGGGAAGTTCTGAGGGGATACCTCGATGACCCGAGGTTCAGAGGGCTTAAAGATGATCCCGAAATC
GTGAGGAGAGCCCTTGAGTTGCTAAAGGCTGAGAAACTTTCACTCTCCCGCTTCAACGACGCCATGATTTTAGTCCGTGC
CATTGAAAAGGGCGTTTTAATAACGTTCGACTCCAAGTTCAGGAAACTGGCCAGAAGGAGGGGTGTTAAAGTCCTCCCGT
AG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10664.54 Da
• Isoelectric Point: 8.5779

>AT237302 WP_072162154.1 NZ_CP092861:c1063187-1062897 [Salmonella enterica]
MKTMPQIAIESNERLSLRVSTDAKKLIVRAAAIQQTNLTDFVVSNVLPVAQKIVDAAERVYLTERDTKMIMEILDNPPAP
NEKLLAAAFALPDMKK

Nucleotide

• Length: 291 bp

>AT237302 NZ_LR881183:c878476-878249 [Thermococcus camini]
ATGAACGTTACCAAAGTAACCCGAAACTACCAGATAACGATTCCGGCGGAGATTAGAAAGGCGCTCGGAATAAGGGAGGG
GGAGCTCCTCGAAGTTCACCTTGAAGGCGACAGGATAGTCCTGAAAAGGCTGGAGAGGAAGAGAAAGAGGCTCAGGATTG
GAAGAAAACTTCCTCCGGAGGAAATAGAGAAGGCCATCGAGGAGGGCATGAAGGAATGCATGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A5Y9PNF0

Antitoxin

• AlphaFold DB: A0A4Y6M833