Detailed information of TA system TA237297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4904407..4905023
• Replicon: chromosome
• Accession: NZ_CP092860
• Organism: Citrobacter braakii strain P1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MJ613_RS23930
• Protein ID: WP_016155182.1
• Coordinates: 4904407..4904781 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: R8WIS4
• Locus tag: MJ613_RS23935
• Protein ID: WP_016155181.1
• Coordinates: 4904781..4905023 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ613_RS23915 (MJ613_23905)	4901910..4902812	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
MJ613_RS23920 (MJ613_23910)	4902809..4903444	+	636	WP_185655067.1	formate dehydrogenase cytochrome b556 subunit	-
MJ613_RS23925 (MJ613_23915)	4903441..4904370	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
MJ613_RS23930 (MJ613_23920)	4904407..4904781	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	Toxin
MJ613_RS23935 (MJ613_23925)	4904781..4905023	-	243	WP_016155181.1	CopG family transcriptional regulator	Antitoxin
MJ613_RS23940 (MJ613_23930)	4905229..4906158	+	930	WP_185655066.1	alpha/beta hydrolase	-
MJ613_RS23945 (MJ613_23935)	4906243..4906554	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	-
MJ613_RS23950 (MJ613_23940)	4906551..4907003	+	453	WP_104651900.1	helix-turn-helix domain-containing protein	-
MJ613_RS23955 (MJ613_23945)	4907021..4907962	-	942	WP_278085725.1	fatty acid biosynthesis protein FabY	-
MJ613_RS23960 (MJ613_23950)	4908007..4908444	-	438	WP_016157874.1	D-aminoacyl-tRNA deacylase	-
MJ613_RS23965 (MJ613_23955)	4908441..4909313	-	873	WP_019077938.1	virulence factor BrkB family protein	-
MJ613_RS23970 (MJ613_23960)	4909307..4909906	-	600	WP_016155174.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13678.84 Da
• Isoelectric Point: 8.5373

>T237297 WP_016155182.1 NZ_CP092860:c4904781-4904407 [Citrobacter braakii]
MVTGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T237297 NZ_LR878366:118250-118648 [Shigella flexneri 3a]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAAAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.72 Da
• Isoelectric Point: 4.3331

>AT237297 WP_016155181.1 NZ_CP092860:c4905023-4904781 [Citrobacter braakii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Nucleotide

• Length: 243 bp

>AT237297 NZ_LR878366:118023-118250 [Shigella flexneri 3a]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCACCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: R8WIS4