Detailed information of TA system TA237296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3869057..3869677
• Replicon: chromosome
• Accession: NZ_CP092860
• Organism: Citrobacter braakii strain P1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: MJ613_RS19180
• Protein ID: WP_002892050.1
• Coordinates: 3869459..3869677 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A7L6U5G9
• Locus tag: MJ613_RS19175
• Protein ID: WP_019076175.1
• Coordinates: 3869057..3869431 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ613_RS19165 (MJ613_19155)	3864204..3865397	+	1194	WP_185654229.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
MJ613_RS19170 (MJ613_19160)	3865420..3868569	+	3150	WP_016152073.1	efflux RND transporter permease AcrB	-
MJ613_RS19175 (MJ613_19165)	3869057..3869431	+	375	WP_019076175.1	Hha toxicity modulator TomB	Antitoxin
MJ613_RS19180 (MJ613_19170)	3869459..3869677	+	219	WP_002892050.1	HHA domain-containing protein	Toxin
MJ613_RS19185 (MJ613_19175)	3869858..3870409	+	552	WP_185654228.1	maltose O-acetyltransferase	-
MJ613_RS19190 (MJ613_19180)	3870526..3870996	+	471	WP_047416865.1	YlaC family protein	-
MJ613_RS19195 (MJ613_19185)	3871075..3871215	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
MJ613_RS19200 (MJ613_19190)	3871217..3871477	-	261	WP_016152070.1	type B 50S ribosomal protein L31	-
MJ613_RS19205 (MJ613_19195)	3871666..3873219	+	1554	WP_185654227.1	EAL domain-containing protein	-
MJ613_RS19210 (MJ613_19200)	3873271..3873624	-	354	WP_003831033.1	DUF1428 family protein	-
MJ613_RS19215 (MJ613_19205)	3873689..3874318	-	630	WP_016155872.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T237296 WP_002892050.1 NZ_CP092860:3869459-3869677 [Citrobacter braakii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T237296 NZ_LR878366:46721-47203 [Shigella flexneri 3a]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATAACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14413.20 Da
• Isoelectric Point: 5.5653

>AT237296 WP_019076175.1 NZ_CP092860:3869057-3869431 [Citrobacter braakii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLVAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT237296 NZ_LR878366:46428-46730 [Shigella flexneri 3a]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2

Antitoxin

• AlphaFold DB: A0A7L6U5G9