Detailed information of TA system TA023728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 369192..369730
• Replicon: chromosome II
• Accession: NC_012583
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q9KMJ0
• Locus tag: VC395_RS16000
• Protein ID: WP_000802136.1
• Coordinates: 369192..369491 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P58093
• Locus tag: VC395_RS16005
• Protein ID: WP_001107719.1
• Coordinates: 369488..369730 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC395_RS20610	364838..364960	+	123	WP_086027501.1	DUF3265 domain-containing protein	-
VC395_RS15965	365128..365310	+	183	WP_000923182.1	DUF645 family protein	-
VC395_RS15970	365510..365983	+	474	WP_001161076.1	GrpB family protein	-
VC395_RS20170	365998..366090	+	93	WP_014378730.1	DUF3265 domain-containing protein	-
VC395_RS15975	366132..366758	+	627	WP_000365423.1	LysE family translocator	-
VC395_RS15980	366881..367579	+	699	WP_001890502.1	hypothetical protein	-
VC395_RS20175	367589..367699	+	111	WP_079857360.1	DUF3265 domain-containing protein	-
VC395_RS15990	368276..368455	+	180	WP_001883058.1	DUF645 family protein	-
VC395_RS15995	368669..369064	+	396	WP_000870857.1	DUF3465 domain-containing protein	-
VC395_RS16000	369192..369491	-	300	WP_000802136.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VC395_RS16005	369488..369730	-	243	WP_001107719.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
VC395_RS19655	369959..370537	+	579	WP_000110120.1	hypothetical protein	-
VC395_RS20190	370559..370651	+	93	WP_014164112.1	DUF3265 domain-containing protein	-
VC395_RS16020	371075..371758	+	684	WP_000877436.1	hypothetical protein	-
VC395_RS16025	371972..372238	+	267	WP_000937852.1	hypothetical protein	-
VC395_RS16030	372393..372992	+	600	WP_000429495.1	hypothetical protein	-
VC395_RS16035	373348..373614	+	267	WP_000937852.1	hypothetical protein	-
VC395_RS16040	373769..374368	+	600	WP_000429495.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	344619..430484	85865

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11589.29 Da
• Isoelectric Point: 9.9232

>T23728 WP_000802136.1 NC_012583:c369491-369192 [Vibrio cholerae O395]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQTGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T23728 NC_012583:c369491-369192 [Vibrio cholerae O395]
ATGAAACCATTTAATCTTACCGTCGCCGCCAAAGCCGATTTACGTGATATTGCTTTATTCACTCAACGACGCTGGGGAAA
AGAGCAGCGAAATGTTTATTTAAAGCAATTCGATGATTCCTTTTGGCTTTTAGCGGAAAATCCCGACATTGGTAAATCAT
GCGATGAAATCCGAGAGGGATACAGAAAATTTCCCCAAGGGAGTCACGTCATCTTTTATCAGCAAACCGGCAGCCAACAA
ATCAGGGTGATCCGAATTCTTCATAAGAGCATGGATGTGAACCCAATATTCGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8964.84 Da
• Isoelectric Point: 4.1677

>AT23728 WP_001107719.1 NC_012583:c369730-369488 [Vibrio cholerae O395]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLIEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT23728 NC_012583:c369730-369488 [Vibrio cholerae O395]
ATGGCTAAAAATACAAGTATCACTCTTGGTGAACACTTCGATGGCTTTATTACAAGCCAAATACAAAGTGGGCGTTACGG
CTCAGCAAGTGAAGTCATTCGCTCTGCGCTACGTCTACTCGAAAACCAAGAAACCAAACTACAGTCACTCCGTCAACTAC
TTATTGAAGGAGAGCAAAGTGGTGACGCTGATTATGACCTTGATAGCTTCATCAATGAACTCGATAGTGAAAACATTCGA
TGA

Structures

Toxin

• PDB: 7R5A

Antitoxin

• AlphaFold DB: A0A151JFU4