Detailed information of TA system TA237273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 85497..86155
• Replicon: plasmid pB1701
• Accession: NZ_CP092854
• Organism: Burkholderia contaminans strain B17-01563-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MMB18_RS38395
• Protein ID: WP_241132228.1
• Coordinates: 85724..86155 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1B4LJE8
• Locus tag: MMB18_RS38390
• Protein ID: WP_043292778.1
• Coordinates: 85497..85724 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMB18_RS38365 (MMB18_38365)	80611..81021	+	411	WP_241132223.1	hypothetical protein	-
MMB18_RS38370 (MMB18_38370)	81025..81393	+	369	WP_241132224.1	hypothetical protein	-
MMB18_RS38375 (MMB18_38375)	81397..83238	+	1842	WP_241132225.1	phage integrase family protein	-
MMB18_RS38380 (MMB18_38380)	83287..83838	-	552	WP_241132226.1	hypothetical protein	-
MMB18_RS38385 (MMB18_38385)	83938..85140	-	1203	WP_241132227.1	hypothetical protein	-
MMB18_RS38390 (MMB18_38390)	85497..85724	+	228	WP_043292778.1	hypothetical protein	Antitoxin
MMB18_RS38395 (MMB18_38395)	85724..86155	+	432	WP_241132228.1	PIN domain-containing protein	Toxin
MMB18_RS38400 (MMB18_38400)	86178..86504	+	327	WP_241132229.1	hypothetical protein	-
MMB18_RS38405 (MMB18_38405)	86626..87156	+	531	WP_241132230.1	PAAR domain-containing protein	-
MMB18_RS38410 (MMB18_38410)	87153..87917	+	765	WP_241132231.1	VRR-NUC domain-containing protein	-
MMB18_RS38415 (MMB18_38415)	87917..89008	+	1092	WP_241132232.1	DUF3396 domain-containing protein	-
MMB18_RS38420 (MMB18_38420)	89025..89576	+	552	WP_241132233.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..182005	182005

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-127)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15515.81 Da
• Isoelectric Point: 7.2341

>T237273 WP_241132228.1 NZ_CP092854:85724-86155 [Burkholderia contaminans]
MAYLLDVNVLIALLDPAHVQHEPAHAWFGRVGHSAWATCPLTENGVLRIIGNPKYPNTMGSPASVAPLVAQLRSHAGHTF
WSDDVSLLDEKHVDATRLLSSAQLTDTYLLALARAHKGKLATFDKRLVVDAVPNGAKHLELIS

Nucleotide

• Length: 432 bp

>T237273 NZ_LR861805:65910-66209 [Xanthomonas sp. CPBF 426]
ATGGCTGAAATAGTCTGGTCGGAGCCGGCGGTGGCCGACCTGGACGCGATTGCCGACTACATTGCACTTGAGGACGCAGC
CGCCGCAGCGGCACTGGTGAGACGGGTGGTTGCGCATGTCGAGCAATTGGCCGAGCACCCGGAGAGCGGGAGCCGCCCGC
AGGAGCTCAAGCGCTCCCGCTACCGTCAAATCGTCGAACCACCGTGCCGCGTGTTCTACCGTGTGGATGGGCAGCGCGTC
GTCGTGGTTCACGTGATGCGCTCGGAGCGGTTACTCCGGAGAAATCGTCTTTCGCGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8080.14 Da
• Isoelectric Point: 8.9793

>AT237273 WP_043292778.1 NZ_CP092854:85497-85724 [Burkholderia contaminans]
MRTTIDIDDDVLAFARERAKPGTSIGKVISDLARAALQRGTTNQSSRNGLPLMPVSATARPVTLDTVNQLRDDSL

Nucleotide

• Length: 228 bp

>AT237273 NZ_LR861805:65665-65922 [Xanthomonas sp. CPBF 426]
ATGCGCACAGAACTTGTCACCACGCTCAAGCGTCAGGCCACCGAACTCCTCGCTGATGTCGAGCGCGACAAGGAGCCGAT
CCTGATTACCCAGCATGGTCTGCCCAGCGCATATCTGGTCGATGTCACCAGCTATGAGCGCCTGCAGCAGCGGATGGCGA
TCCTCGAAGGTATCGCCCGTGGTGAAATGGCGGTAGCGGAAGGCCGCAGCCTGAGCCACGAGCAGGCCAAACAACGCCTG
GCGCGATGGCTGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A1B4LJE8