Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 361241..362007 Replicon chromosome II
Accession NC_012583
Organism Vibrio cholerae O395

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag VC395_RS15930 Protein ID WP_000982260.1
Coordinates 361241..361738 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag VC395_RS15935 Protein ID WP_000246253.1
Coordinates 361735..362007 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VC395_RS15895 356511..356993 + 483 WP_001895003.1 hypothetical protein -
VC395_RS15905 357440..358315 + 876 WP_000259044.1 hypothetical protein -
VC395_RS15910 358445..358900 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
VC395_RS21110 358957..359052 + 96 WP_080368267.1 acetyltransferase -
VC395_RS15915 359084..359497 + 414 WP_000049417.1 VOC family protein -
VC395_RS15920 359648..360082 + 435 WP_000453957.1 GNAT family N-acetyltransferase -
VC395_RS20150 360868..361038 + 171 WP_000483445.1 DUF645 family protein -
VC395_RS15930 361241..361738 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
VC395_RS15935 361735..362007 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
VC395_RS15940 362397..362579 + 183 WP_000947517.1 DUF645 family protein -
VC395_RS15945 362793..363188 + 396 WP_000870841.1 DUF3465 domain-containing protein -
VC395_RS15950 363380..363703 + 324 WP_001889156.1 DUF3709 domain-containing protein -
VC395_RS15960 364132..364311 + 180 WP_001889214.1 DUF645 family protein -
VC395_RS20610 364838..364960 + 123 WP_086027501.1 DUF3265 domain-containing protein -
VC395_RS15965 365128..365310 + 183 WP_000923182.1 DUF645 family protein -
VC395_RS15970 365510..365983 + 474 WP_001161076.1 GrpB family protein -
VC395_RS20170 365998..366090 + 93 WP_014378730.1 DUF3265 domain-containing protein -
VC395_RS15975 366132..366758 + 627 WP_000365423.1 LysE family translocator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - - 344619..430484 85865


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T23727 WP_000982260.1 NC_012583:c361738-361241 [Vibrio cholerae O395]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T23727 NC_012583:c361738-361241 [Vibrio cholerae O395]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT23727 WP_000246253.1 NC_012583:c362007-361735 [Vibrio cholerae O395]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT23727 NC_012583:c362007-361735 [Vibrio cholerae O395]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
PDB 1Y9B
AlphaFold DB A0A0F2IC79

References