Detailed information of TA system TA237256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1854702..1855304
• Replicon: chromosome
• Accession: NZ_CP092844
• Organism: Burkholderia ambifaria strain Q53

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A6P2QRH5
• Locus tag: MK974_RS24260
• Protein ID: WP_059771351.1
• Coordinates: 1854702..1854884 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MK974_RS24265
• Protein ID: WP_269017324.1
• Coordinates: 1854909..1855304 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MK974_RS24235 (MK974_24210)	1850500..1851759	-	1260	WP_269017319.1	phage portal protein	-
MK974_RS24240 (MK974_24215)	1851764..1851949	-	186	WP_269017320.1	hypothetical protein	-
MK974_RS24245 (MK974_24220)	1851946..1853658	-	1713	WP_269017321.1	terminase large subunit	-
MK974_RS24250 (MK974_24225)	1853668..1854153	-	486	WP_269017322.1	phage terminase small subunit P27 family	-
MK974_RS24255 (MK974_24230)	1854304..1854660	-	357	WP_269017323.1	HNH endonuclease	-
MK974_RS24260 (MK974_24235)	1854702..1854884	+	183	WP_059771351.1	type II toxin-antitoxin system HicA family toxin	Toxin
MK974_RS24265 (MK974_24240)	1854909..1855304	+	396	WP_269017324.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MK974_RS24270 (MK974_24245)	1855314..1855952	+	639	WP_269017325.1	hypothetical protein	-
MK974_RS24275 (MK974_24250)	1856027..1856617	-	591	WP_269017326.1	DNA-binding protein	-
MK974_RS24280 (MK974_24255)	1856661..1856909	-	249	WP_269017327.1	phage portal protein	-
MK974_RS24285 (MK974_24260)	1856906..1857238	-	333	WP_269017328.1	hypothetical protein	-
MK974_RS24290 (MK974_24265)	1857241..1857684	-	444	WP_269017329.1	DUF1364 family protein	-
MK974_RS24295 (MK974_24270)	1857681..1858082	-	402	WP_269017330.1	winged helix-turn-helix domain-containing protein	-
MK974_RS24300 (MK974_24275)	1858420..1858893	-	474	WP_269018491.1	RusA family crossover junction endodeoxyribonuclease	-
MK974_RS24305 (MK974_24280)	1859255..1859902	-	648	WP_269017331.1	DUF6475 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1823309..1877970	54661

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-123)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6774.99 Da
• Isoelectric Point: 11.1039

>T237256 WP_059771351.1 NZ_CP092844:1854702-1854884 [Burkholderia ambifaria]
MNSTELIKRIKADGWYHVHTVGSHHQFKHPTKPGKVTVPHPKKDLLIATVRSILKQAGLK

Nucleotide

• Length: 183 bp

>T237256 NZ_LR822061:2869805-2870014 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14285.10 Da
• Isoelectric Point: 4.7231

>AT237256 WP_269017324.1 NZ_CP092844:1854909-1855304 [Burkholderia ambifaria]
MLYPLYVHVGDAKHAHGVTFPDFPGCFAAADTWEDLPAAVQEAVEAHFYGEEGAVPSPSALETLARNPEYEGGVWMLFDI
DLSKINSKAVRFNVSMPERLLQQIDAAANARKLSRSAFLALAAEHEMSAHA

Nucleotide

• Length: 396 bp

>AT237256 NZ_LR822061:2869500-2869793 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A6P2QRH5