Detailed information of TA system TA237255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1768526..1769172
• Replicon: chromosome
• Accession: NZ_CP092844
• Organism: Burkholderia ambifaria strain Q53

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MK974_RS23795
• Protein ID: WP_269017262.1
• Coordinates: 1768526..1768933 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q0BA09
• Locus tag: MK974_RS23800
• Protein ID: WP_011658485.1
• Coordinates: 1768930..1769172 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MK974_RS23775 (MK974_23750)	1764308..1764892	-	585	WP_269017258.1	hypothetical protein	-
MK974_RS23780 (MK974_23755)	1764889..1765629	-	741	WP_269017259.1	methyl-accepting chemotaxis protein	-
MK974_RS23785 (MK974_23760)	1765632..1768022	-	2391	WP_269017260.1	MCP four helix bundle domain-containing protein	-
MK974_RS23790 (MK974_23765)	1768080..1768394	+	315	WP_269017261.1	hypothetical protein	-
MK974_RS23795 (MK974_23770)	1768526..1768933	-	408	WP_269017262.1	type II toxin-antitoxin system VapC family toxin	Toxin
MK974_RS23800 (MK974_23775)	1768930..1769172	-	243	WP_011658485.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MK974_RS23805 (MK974_23780)	1769433..1770131	+	699	WP_269017263.1	phospholipase	-
MK974_RS23810 (MK974_23785)	1770176..1771669	-	1494	WP_269017264.1	amino acid permease	-
MK974_RS23815 (MK974_23790)	1771773..1773179	-	1407	WP_175662983.1	aspartate ammonia-lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14854.90 Da
• Isoelectric Point: 6.0877

>T237255 WP_269017262.1 NZ_CP092844:c1768933-1768526 [Burkholderia ambifaria]
MKFLLDTNAVIAILKGEQAMLARLRSHQPADFGIPAIVAHELYYGAYKSQRAAANLARVEGLQFEVVSFDAEDAQHAGEI
RAQLAAVGTPIGPYDALIAGQARARRLVLVTHNTREFERISELQVEDWLAAAREN

Nucleotide

• Length: 408 bp

>T237255 NZ_LR822061:2786781-2787017 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATCGAACAGTCCGTTATAAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGATTACTAAA
AGAGATTGAAGACGTGTATAAGAAAGCACAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTATTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8872.03 Da
• Isoelectric Point: 4.7628

>AT237255 WP_011658485.1 NZ_CP092844:c1769172-1768930 [Burkholderia ambifaria]
METAKIFKHGGSQAVRLPKDFRFDTTEVRIRRHGASVILEPVPQDWAWLTPLIGPVDADFEAAAATEPAAQERPGLDVFE

Nucleotide

• Length: 243 bp

>AT237255 NZ_LR822061:2787022-2787204 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGCACAGGAAAATGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A3P0NDJ4