Detailed information of TA system TA023725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-Phd
• Location: 349302..349880
• Replicon: chromosome II
• Accession: NC_012583
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: parE
• Uniprot ID: O68848
• Locus tag: VC395_RS15835
• Protein ID: WP_001180243.1
• Coordinates: 349302..349619 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q7DCR7
• Locus tag: VC395_RS15840
• Protein ID: WP_000557292.1
• Coordinates: 349638..349880 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC395_RS15810	344619..345581	-	963	WP_000841995.1	integron integrase IntIA	-
VC395_RS15815	345826..346425	+	600	WP_000429496.1	hypothetical protein	-
VC395_RS15820	346704..347303	+	600	WP_000429495.1	hypothetical protein	-
VC395_RS15825	347589..348272	+	684	WP_000877436.1	hypothetical protein	-
VC395_RS21030	349013..349108	+	96	WP_012706049.1	DUF645 family protein	-
VC395_RS15835	349302..349619	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VC395_RS15840	349638..349880	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
VC395_RS15845	350122..350631	+	510	WP_000405986.1	GNAT family N-acetyltransferase	-
VC395_RS15850	350688..351341	+	654	WP_000226874.1	hypothetical protein	-
VC395_RS15855	351651..351869	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
VC395_RS15860	352272..352505	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
VC395_RS15865	352930..353110	+	181	Protein_313	DUF645 family protein	-
VC395_RS15870	353377..353664	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VC395_RS15875	353675..353992	+	318	WP_001232701.1	HigA family addiction module antidote protein	-
VC395_RS21035	353989..354099	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
VC395_RS20120	354276..354401	+	126	WP_001944767.1	DUF645 family protein	-
VC395_RS20805	354417..354455	+	39	WP_106019119.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	344619..430484	85865

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-93)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12158.97 Da
• Isoelectric Point: 9.7495

>T23725 WP_001180243.1 NC_012583:c349619-349302 [Vibrio cholerae O395]
MQNKQYKLSQLAQEHLLKIKHYTIENFAEAQWQKYKSTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS

Nucleotide

• Length: 318 bp

>T23725 NC_012583:c349619-349302 [Vibrio cholerae O395]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8961.25 Da
• Isoelectric Point: 5.6630

>AT23725 WP_000557292.1 NC_012583:c349880-349638 [Vibrio cholerae O395]
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDSGKTVDGATFLNTL

Nucleotide

• Length: 243 bp

>AT23725 NC_012583:c349880-349638 [Vibrio cholerae O395]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA

Structures

Toxin

• AlphaFold DB: A0A0K9UJR8

Antitoxin

• AlphaFold DB: Q7DCR7