Detailed information of TA system TA237248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1755847..1756532
• Replicon: chromosome
• Accession: NZ_CP092843
• Organism: Burkholderia ambifaria strain Q53

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q0BFH0
• Locus tag: MK974_RS08040
• Protein ID: WP_011656832.1
• Coordinates: 1756110..1756532 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: MK974_RS08035
• Protein ID: WP_175737985.1
• Coordinates: 1755847..1756113 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MK974_RS08020 (MK974_08025)	1751306..1753627	+	2322	WP_269015474.1	TonB-dependent siderophore receptor	-
MK974_RS08025 (MK974_08030)	1753714..1754553	+	840	WP_006755049.1	formyltransferase family protein	-
MK974_RS08030 (MK974_08035)	1754553..1755569	+	1017	WP_269015477.1	GNAT family N-acetyltransferase	-
MK974_RS08035 (MK974_08040)	1755847..1756113	+	267	WP_175737985.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MK974_RS08040 (MK974_08045)	1756110..1756532	+	423	WP_011656832.1	type II toxin-antitoxin system VapC family toxin	Toxin
MK974_RS08045 (MK974_08050)	1756575..1757480	-	906	WP_269015481.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MK974_RS08050 (MK974_08055)	1757449..1758540	-	1092	WP_269015482.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
MK974_RS08055 (MK974_08060)	1758875..1760038	+	1164	WP_175655748.1	M20 aminoacylase family protein	-
MK974_RS08060 (MK974_08065)	1760060..1761385	-	1326	WP_175679897.1	cobyrinate a,c-diamide synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14774.86 Da
• Isoelectric Point: 7.8895

>T237248 WP_011656832.1 NZ_CP092843:1756110-1756532 [Burkholderia ambifaria]
VTTLYMLDTNICSFIMRERPSAVLSRLQSCVNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVSRLDGVLSWDTA
AVDATTAIRVDLAARGTPIGANDASIAGHAIAAGAVLVTNNSREFSRVAGLSLEDWAASA

Nucleotide

• Length: 423 bp

>T237248 NZ_LR822061:516140-516376 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATCGAACAGTCCGTTATAAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGATTACTAAA
AGAGATTGAAGACGTGTATAAGAAAGCACAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTATTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9986.27 Da
• Isoelectric Point: 4.5498

>AT237248 WP_175737985.1 NZ_CP092843:1755847-1756113 [Burkholderia ambifaria]
MRTVSIFKNARNQAIRIPKDMEFEGVTELEIRREGDTLLLRPVRPTWLSFATEPRADADFLVERPTVVEAGRFELPGADD
DAPTESAR

Nucleotide

• Length: 267 bp

>AT237248 NZ_LR822061:516381-516563 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGCACAGGAAAATGACTAA

Structures

Toxin

• AlphaFold DB: Q0BFH0