Detailed information of TA system TA237246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 86189..86991
• Replicon: plasmid p1
• Accession: NZ_CP092842
• Organism: Bradyrhizobium sp. 62B

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: ML401_RS35310
• Protein ID: WP_285813124.1
• Coordinates: 86464..86991 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: ML401_RS35305
• Protein ID: WP_285813123.1
• Coordinates: 86189..86467 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ML401_RS35295 (ML401_35195)	82108..84762	+	2655	WP_285813122.1	PAS domain S-box protein	-
ML401_RS35300 (ML401_35200)	85029..86036	-	1008	Protein_79	site-specific integrase	-
ML401_RS35305 (ML401_35205)	86189..86467	+	279	WP_285813123.1	DUF1778 domain-containing protein	Antitoxin
ML401_RS35310 (ML401_35210)	86464..86991	+	528	WP_285813124.1	GNAT family N-acetyltransferase	Toxin
ML401_RS35315 (ML401_35215)	87274..87570	+	297	WP_285813125.1	DUF1403 family protein	-
ML401_RS35320 (ML401_35220)	87713..88111	+	399	WP_285813126.1	DUF1403 family protein	-
ML401_RS35325 (ML401_35225)	88113..88763	+	651	WP_285813127.1	SMC-Scp complex subunit ScpB	-
ML401_RS35330	88805..88927	-	123	WP_285813128.1	hypothetical protein	-
ML401_RS35335 (ML401_35230)	88927..89112	-	186	WP_285813129.1	hypothetical protein	-
ML401_RS35340	89330..89464	-	135	WP_285813130.1	hypothetical protein	-
ML401_RS35345 (ML401_35235)	89461..90006	-	546	WP_285813131.1	hypothetical protein	-
ML401_RS35350 (ML401_35240)	90185..90348	+	164	Protein_89	ATP-binding cassette domain-containing protein	-
ML401_RS35355 (ML401_35245)	90570..90707	+	138	Protein_90	cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..859431	859431
	-	flank	IS/Tn	-	-	88805..88927	122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (11-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18706.64 Da
• Isoelectric Point: 9.1173

>T237246 WP_285813124.1 NZ_CP092842:86464-86991 [Bradyrhizobium sp. 62B]
VTVPAWHEEPIAKSHDRETFDCGDDALNTFLMRYARQGHEQNAVKTYCAIANDAPSLVLGFYSVAPASVAHKAVAAVMTK
GLARHKVPGFLLARLAVDKTVAGRGLGGQLLLAAALRCLRVTEEIGGVLIGIEAKNERAAQWYRGFGAEPLAYRPLTLVA
PLATFADALRAAGRL

Nucleotide

• Length: 528 bp

>T237246 NZ_LR822061:123101-123253 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10146.82 Da
• Isoelectric Point: 10.4689

>AT237246 WP_285813123.1 NZ_CP092842:86189-86467 [Bradyrhizobium sp. 62B]
MARVAIDDTNRMNLRIKPEVKARLMRAAALRHTDLTSFVTQSALREADAVIAEADVIKVPERDFARILDLLDNPPKANAK
LRAAAASLPKDL

Nucleotide

• Length: 279 bp

>AT237246 NZ_LR822061:122477-123076 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA