Detailed information of TA system TA237245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2423982..2424960
• Replicon: chromosome
• Accession: NZ_CP092833
• Organism: Bacillus mycoides strain LX30

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: MLD62_RS12390
• Protein ID: WP_085311113.1
• Coordinates: 2423982..2424722 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: MLD62_RS12395
• Protein ID: WP_000588712.1
• Coordinates: 2424835..2424960 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLD62_RS12370	2420549..2421259	+	711	WP_085311106.1	class I SAM-dependent methyltransferase	-
MLD62_RS12375	2421378..2421791	+	414	WP_085311108.1	VOC family protein	-
MLD62_RS12380	2422280..2422756	+	477	WP_085311110.1	MarR family winged helix-turn-helix transcriptional regulator	-
MLD62_RS12385	2422910..2423647	+	738	WP_241139070.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
MLD62_RS12390	2423982..2424722	+	741	WP_085311113.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MLD62_RS12395	2424835..2424960	+	126	WP_000588712.1	hypothetical protein	Antitoxin
MLD62_RS12400	2425036..2425212	+	177	WP_170972692.1	stage II sporulation protein SB	-
MLD62_RS12405	2425232..2425621	-	390	WP_085312972.1	YxeA family protein	-
MLD62_RS12410	2425874..2427343	+	1470	WP_016102002.1	aminoacyl-histidine dipeptidase	-
MLD62_RS12415	2427703..2427852	+	150	WP_002069316.1	hypothetical protein	-
MLD62_RS12420	2428608..2429267	+	660	WP_085311123.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28471.19 Da
• Isoelectric Point: 8.2847

>T237245 WP_085311113.1 NZ_CP092833:2423982-2424722 [Bacillus mycoides]
MTISNIRIGLFILVIAFVVLVFFYWRNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWQTEEEYTKSLNAFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Nucleotide

• Length: 741 bp

>T237245 NZ_LR822061:18786-18962 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT237245 WP_000588712.1 NZ_CP092833:2424835-2424960 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT237245 NZ_LR822061:c19124-18950 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Antitoxin

• AlphaFold DB: A0A366GQT1