Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 241263..241905 Replicon chromosome
Accession NZ_CP092833
Organism Bacillus mycoides strain LX30

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag MLD62_RS01355 Protein ID WP_000635965.1
Coordinates 241555..241905 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag MLD62_RS01350 Protein ID WP_000004570.1
Coordinates 241263..241550 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MLD62_RS01325 236579..237541 + 963 WP_002029436.1 UV DNA damage repair endonuclease UvsE -
MLD62_RS01330 237534..238106 - 573 WP_002167128.1 rhomboid family intramembrane serine protease -
MLD62_RS01335 238198..238557 + 360 WP_002009969.1 holo-ACP synthase -
MLD62_RS01340 238714..239664 + 951 WP_241139139.1 outer membrane lipoprotein carrier protein LolA -
MLD62_RS01345 239782..240951 + 1170 WP_085308684.1 alanine racemase -
MLD62_RS01350 241263..241550 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
MLD62_RS01355 241555..241905 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MLD62_RS01360 241974..244142 + 2169 WP_085312924.1 Tex family protein -
MLD62_RS01365 244200..244316 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
MLD62_RS01370 244527..244982 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T237244 WP_000635965.1 NZ_CP092833:241555-241905 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T237244 NZ_LR822060:2889576-2889938 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT237244 WP_000004570.1 NZ_CP092833:241263-241550 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT237244 NZ_LR822060:2889409-2889579 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References