Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 536779..537415 Replicon chromosome
Accession NZ_CP092829
Organism Bacillus pumilus strain MS32

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag MK860_RS02585 Protein ID WP_003214169.1
Coordinates 537065..537415 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag MK860_RS02580 Protein ID WP_003214273.1
Coordinates 536779..537060 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MK860_RS02560 (MK860_02555) 532925..533530 - 606 WP_265209109.1 rhomboid family intramembrane serine protease -
MK860_RS02565 (MK860_02560) 533625..533990 + 366 WP_044140566.1 holo-ACP synthase -
MK860_RS02570 (MK860_02565) 534151..535167 + 1017 WP_113754968.1 outer membrane lipoprotein carrier protein LolA -
MK860_RS02575 (MK860_02570) 535303..536484 + 1182 WP_113768718.1 alanine racemase -
MK860_RS02580 (MK860_02575) 536779..537060 + 282 WP_003214273.1 hypothetical protein Antitoxin
MK860_RS02585 (MK860_02580) 537065..537415 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MK860_RS02590 (MK860_02585) 537529..538359 + 831 WP_044140569.1 RsbT co-antagonist protein RsbRA -
MK860_RS02595 (MK860_02590) 538364..538732 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
MK860_RS02600 (MK860_02595) 538735..539136 + 402 WP_003214085.1 anti-sigma regulatory factor -
MK860_RS02605 (MK860_02600) 539147..540154 + 1008 WP_065097543.1 PP2C family protein-serine/threonine phosphatase -
MK860_RS02610 (MK860_02605) 540214..540543 + 330 WP_044140572.1 anti-sigma factor antagonist -
MK860_RS02615 (MK860_02610) 540540..541028 + 489 WP_044140573.1 anti-sigma B factor RsbW -
MK860_RS02620 (MK860_02615) 540994..541782 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
MK860_RS02625 (MK860_02620) 541782..542381 + 600 WP_044140574.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T237238 WP_003214169.1 NZ_CP092829:537065-537415 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T237238 NZ_LR822060:2450295-2450561 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT237238 WP_003214273.1 NZ_CP092829:536779-537060 [Bacillus pumilus]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT237238 NZ_LR822060:2450038-2450295 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References