Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3721172..3721748 Replicon chromosome
Accession NZ_CP092828
Organism Klebsiella michiganensis strain HOBD-7

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag MJ905_RS17505 Protein ID WP_255850733.1
Coordinates 3721172..3721459 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1D8JNF7
Locus tag MJ905_RS17510 Protein ID WP_025108476.1
Coordinates 3721446..3721748 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ905_RS17475 (MJ905_17470) 3716512..3717030 - 519 WP_004098240.1 FidL-like protein -
MJ905_RS17480 (MJ905_17475) 3717027..3717827 - 801 WP_014837308.1 winged helix-turn-helix domain-containing protein -
MJ905_RS17485 (MJ905_17480) 3717973..3718557 - 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
MJ905_RS17490 (MJ905_17485) 3718705..3719487 - 783 WP_255850345.1 NAD(P)H-dependent oxidoreductase -
MJ905_RS17495 (MJ905_17490) 3719575..3720483 + 909 WP_119834582.1 LysR family transcriptional regulator -
MJ905_RS17500 (MJ905_17495) 3720477..3720920 + 444 WP_014227755.1 FosA family fosfomycin resistance glutathione transferase -
MJ905_RS17505 (MJ905_17500) 3721172..3721459 + 288 WP_255850733.1 BrnT family toxin Toxin
MJ905_RS17510 (MJ905_17505) 3721446..3721748 + 303 WP_025108476.1 BrnA antitoxin family protein Antitoxin
MJ905_RS17515 (MJ905_17510) 3721870..3723213 - 1344 WP_049101852.1 APC family permease -
MJ905_RS17520 (MJ905_17515) 3723210..3724121 - 912 WP_255850346.1 acetamidase/formamidase family protein -
MJ905_RS17525 (MJ905_17520) 3724574..3724909 - 336 WP_255850347.1 endoribonuclease SymE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11141.58 Da        Isoelectric Point: 6.9902

>T237235 WP_255850733.1 NZ_CP092828:3721172-3721459 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVFDDPRHLSRQDSYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

>T237235 NZ_LR822060:514808-515044 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATCGAACAGTCCGTTATAAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGATTACTAAA
AGAGATTGAAGACGTGTATAAGAAAGCACAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTATTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAATAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11563.17 Da        Isoelectric Point: 9.7962

>AT237235 WP_025108476.1 NZ_CP092828:3721446-3721748 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRADELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT237235 NZ_LR822060:515049-515231 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGCACAGGAAAATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JNF7

References