Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2942789..2943336 Replicon chromosome
Accession NZ_CP092828
Organism Klebsiella michiganensis strain HOBD-7

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag MJ905_RS13895 Protein ID WP_014227299.1
Coordinates 2943028..2943336 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag MJ905_RS13890 Protein ID WP_014227298.1
Coordinates 2942789..2943025 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ905_RS13875 (MJ905_13870) 2938996..2940162 + 1167 WP_042946137.1 multidrug effflux MFS transporter -
MJ905_RS13880 (MJ905_13875) 2940323..2941225 + 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
MJ905_RS13885 (MJ905_13880) 2941250..2942683 + 1434 WP_032718833.1 PTS N-acetylmuramic acid transporter subunit IIBC -
MJ905_RS13890 (MJ905_13885) 2942789..2943025 + 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MJ905_RS13895 (MJ905_13890) 2943028..2943336 + 309 WP_014227299.1 CcdB family protein Toxin
MJ905_RS13900 (MJ905_13895) 2943487..2944389 + 903 WP_014227300.1 EamA family transporter -
MJ905_RS13905 (MJ905_13900) 2944393..2944650 - 258 WP_042946138.1 hypothetical protein -
MJ905_RS13910 (MJ905_13905) 2944650..2945615 - 966 WP_038423881.1 Rpn family recombination-promoting nuclease/putative transposase -
MJ905_RS13915 (MJ905_13910) 2945825..2946118 + 294 WP_014227302.1 YicS family protein -
MJ905_RS13920 (MJ905_13915) 2946142..2947083 - 942 WP_014227303.1 LacI family DNA-binding transcriptional regulator -
MJ905_RS13925 (MJ905_13920) 2947378..2947689 + 312 WP_014227304.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T237233 WP_014227299.1 NZ_CP092828:2943028-2943336 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T237233 NZ_LR822060:123101-123253 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT237233 WP_014227298.1 NZ_CP092828:2942789-2943025 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT237233 NZ_LR822060:122477-123076 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References