Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 951211..951801 Replicon chromosome
Accession NZ_CP092828
Organism Klebsiella michiganensis strain HOBD-7

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag MJ905_RS04525 Protein ID WP_014229979.1
Coordinates 951211..951543 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag MJ905_RS04530 Protein ID WP_004852307.1
Coordinates 951544..951801 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ905_RS04500 (MJ905_04495) 946624..946881 + 258 WP_014229908.1 histidine kinase -
MJ905_RS04505 (MJ905_04500) 947014..948468 + 1455 WP_014229909.1 AMP nucleosidase -
MJ905_RS04515 (MJ905_04510) 949431..950888 - 1458 WP_014229916.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
MJ905_RS04525 (MJ905_04520) 951211..951543 - 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MJ905_RS04530 (MJ905_04525) 951544..951801 - 258 WP_004852307.1 antitoxin Antitoxin
MJ905_RS04535 (MJ905_04530) 952512..952592 + 81 Protein_890 molybdopterin-binding protein -
MJ905_RS04540 (MJ905_04535) 952783..953697 - 915 WP_014229981.1 LysR family transcriptional regulator -
MJ905_RS04545 (MJ905_04540) 953798..954796 + 999 WP_014229982.1 aldo/keto reductase -
MJ905_RS04550 (MJ905_04545) 954834..955760 - 927 WP_014229983.1 LysR substrate-binding domain-containing protein -
MJ905_RS04555 (MJ905_04550) 955940..956545 + 606 WP_032750440.1 glutathione S-transferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T237230 WP_014229979.1 NZ_CP092828:c951543-951211 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T237230 NZ_LR822027:c697084-696248 [Streptococcus thermophilus]
GTGAACAAAGCAAAATTAACGGCACTCTGCCATAAAGTTTCAAAAGAAATAGGATTAACGTTTAATTCTGTGATGACCTA
CTATTTTCTGGAAATTATTCTGAAAAAATTGAGCCAAAGCGCTTATGCTCAAAATTACATTTTCAAAGGAGGTTTTCTGC
TCTCAAATGTCGTCGGGGTTGCTTCCCGTAGTACCGTTGACATTGACTTTCTCTTTAGTAAAATGAATCTATCAGAAGAG
ACCGTGCATCAGCAATTGGAGGATATCCTTGGAAAATCTGATGAAGATATTTCTTTCACTATTCAGTCGGTCACAGCAAT
TAAGGAAAGTGATGATTACGGAGGTTATCGTGCAAGTATCCTTTGCCAGCTTGAGAACATCAGACAGGTCATCCACCTAG
ATATTGCGACTGGGGACATTGTCACTCCGCAGCCCATCACTTACGACTACAAGCCAATCTTCACATCAGAACAGTTTCCT
ATCATTGCCTACACGATTGAGACTATCTTAGCAGAGAAACTACAAACTATTTATTCTCGCAGCTTCTTGAATAGTCGAAG
CAAAGACTTCTATGACGTCTATATCTTGTCAAAATTGAAGAAATCAGAAATTGATTCGCAACAACTTCTCACTGCTTGTC
AGTATACCTTCGATTATCGAGGTACTGAATTAGACTTCGATAAATTCATCAATCTGCTAGAAACATTCAAAACAGATTCA
ACCCAAAACCATCAGTGGGAAAACTACTCTAAAAAGTATAGTTACACTAAAGGTATTACATTTAACGATGTTCTGACAGA
GATGACTGATTTGCTTGTAACTATCAAAAATCTCTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT237230 WP_004852307.1 NZ_CP092828:c951801-951544 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT237230 NZ_LR822027:c697671-697081 [Streptococcus thermophilus]
ATGTCTAATAAAGAAACCGTTCTGAATTATCTCAAACAGAATAACGGTATCATTACATATAAAGACTGTAAAGAGCTTGG
GGTTCCAGCTGTCTATCTCTCACGACTTGAAAATGAAGAGAAAATATTCCGAGTGGACAAGGGCATCTATCTTTCTACCG
ATGGTGACTATGACGAGTACTACTTCTTCCAATACAGATTTTCAAGAGCTATTTACTCCTATGTTTCCGCTCTTTATCTT
CAAGGGCTCACGGATGAAATCCCTCAATATTTTGAAGTCACCGTACCAAGAGGGTATCGTTTCAATAATCCGCCTCAAAA
CCTTGCGGTTCATACCGTCTCAAAGGAAATTGTTGACCTTGGTGTTATCTCTGTAAAGACACCCTGGGGACAGACCGTTA
AGACCTATGACTTTGAGCGTATCATCTGTGACTTTATTCAAAATCGTAAAAAAATTGATAATGAACTTTTCGTCAAAACT
CTTAAGGCTTATGTCAACTATCCAAATAAAAACCTGACAAACCTTTACCAATACTCTGAAAAAATGAAAATTATCAATCA
AACAAAACAAACACTTGAGGTGCTAATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References