Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 69..651 Replicon plasmid pXH1507-4
Accession NZ_CP092797
Organism Klebsiella pneumoniae strain XH1507

Toxin (Protein)


Gene name PumA Uniprot ID B1VJ82
Locus tag ML258_RS29495 Protein ID WP_012368903.1
Coordinates 69..365 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID B1VJ81
Locus tag ML258_RS29500 Protein ID WP_012368902.1
Coordinates 367..651 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ML258_RS29495 (ML258_29495) 69..365 + 297 WP_012368903.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ML258_RS29500 (ML258_29500) 367..651 + 285 WP_012368902.1 putative addiction module antidote protein Antitoxin
ML258_RS29505 (ML258_29505) 1359..1907 + 549 WP_212656696.1 TraY domain protein -
ML258_RS29510 (ML258_29510) 1915..3075 + 1161 WP_212656697.1 MobP1 family relaxase -
ML258_RS29515 (ML258_29515) 3085..3441 + 357 WP_212656698.1 DNA distortion polypeptide 3 -
ML258_RS29520 (ML258_29520) 3418..3942 - 525 WP_212656699.1 restriction endonuclease -
ML258_RS29525 (ML258_29525) 4034..4363 - 330 WP_113355598.1 hypothetical protein -
ML258_RS29530 (ML258_29530) 4363..4608 - 246 WP_113355596.1 hypothetical protein -
ML258_RS29535 (ML258_29535) 4908..5189 - 282 WP_113355595.1 CopG family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..34012 34012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Uncharacterized protein HI1420

(3-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11325.21 Da        Isoelectric Point: 10.2910

>T237198 WP_012368903.1 NZ_CP092797:69-365 [Klebsiella pneumoniae]
MIEIRQTEEVKKWLKGLKDKIAKAKILTRIERMKEGNYGDVEPIGDGYSELRIHQGKGYRVYFANRNNEIILLLCGGDKT
TQQADIKKAKQIAKKWGF

Download         Length: 297 bp

>T237198 NZ_LR792628:927931-928341 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10302.61 Da        Isoelectric Point: 4.6286

>AT237198 WP_012368902.1 NZ_CP092797:367-651 [Klebsiella pneumoniae]
MKTKPELFDVVDYLEDEEDIQAYLNAALDEDDESYFLAALGNIARARNISQLSKETGMSREGIYKALSGTGNPSFKTISK
ISKALGLKIHFAGA

Download         Length: 285 bp

>AT237198 NZ_LR792628:927684-927950 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I3XCB7

Antitoxin

Source ID Structure
AlphaFold DB A0A1I3XCJ1

References