Detailed information of TA system TA237193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 124164..124834
• Replicon: plasmid pXH1507-1
• Accession: NZ_CP092794
• Organism: Klebsiella pneumoniae strain XH1507

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: ML258_RS27745
• Protein ID: WP_004213072.1
• Coordinates: 124164..124607 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: ML258_RS27750
• Protein ID: WP_004213073.1
• Coordinates: 124604..124834 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ML258_RS27710 (ML258_27710)	119575..119850	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
ML258_RS27715 (ML258_27715)	119913..120404	+	492	WP_011251327.1	DM13 domain-containing protein	-
ML258_RS27720 (ML258_27720)	120453..121373	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
ML258_RS27725 (ML258_27725)	121464..121867	+	404	Protein_140	GAF domain-containing protein	-
ML258_RS27730 (ML258_27730)	122385..123020	-	636	Protein_141	mucoid phenotype regulator RmpA2	-
ML258_RS27735 (ML258_27735)	123437..123741	+	305	Protein_142	transposase	-
ML258_RS27740 (ML258_27740)	123764..124015	-	252	WP_186987481.1	hypothetical protein	-
ML258_RS27745 (ML258_27745)	124164..124607	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
ML258_RS27750 (ML258_27750)	124604..124834	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
ML258_RS27755 (ML258_27755)	125442..126575	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
ML258_RS27760 (ML258_27760)	126591..126884	+	294	WP_004213076.1	hypothetical protein	-
ML258_RS27765 (ML258_27765)	126874..127080	-	207	WP_004213077.1	hypothetical protein	-
ML258_RS27770 (ML258_27770)	127432..127722	+	291	WP_004213078.1	hypothetical protein	-
ML258_RS27775 (ML258_27775)	127712..128611	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..198615	198615

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T237193 WP_004213072.1 NZ_CP092794:c124607-124164 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T237193 NZ_LR782232:c4535219-4535046 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT237193 WP_004213073.1 NZ_CP092794:c124834-124604 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT237193 NZ_LR782232:c4535543-4535247 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620