Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5390887..5391512 Replicon chromosome
Accession NZ_CP092793
Organism Klebsiella pneumoniae strain XH1507

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ML258_RS26660 Protein ID WP_002882817.1
Coordinates 5390887..5391270 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ML258_RS26665 Protein ID WP_004150355.1
Coordinates 5391270..5391512 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ML258_RS26645 (ML258_26645) 5388253..5389155 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ML258_RS26650 (ML258_26650) 5389152..5389787 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ML258_RS26655 (ML258_26655) 5389784..5390713 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ML258_RS26660 (ML258_26660) 5390887..5391270 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ML258_RS26665 (ML258_26665) 5391270..5391512 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
ML258_RS26670 (ML258_26670) 5391717..5392634 + 918 WP_002882812.1 alpha/beta hydrolase -
ML258_RS26675 (ML258_26675) 5392648..5393589 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ML258_RS26680 (ML258_26680) 5393634..5394071 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
ML258_RS26685 (ML258_26685) 5394068..5394928 - 861 WP_002882807.1 virulence factor BrkB family protein -
ML258_RS26690 (ML258_26690) 5394922..5395521 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T237189 WP_002882817.1 NZ_CP092793:c5391270-5390887 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T237189 NZ_LR782232:3720027-3720245 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT237189 WP_004150355.1 NZ_CP092793:c5391512-5391270 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT237189 NZ_LR782232:3719627-3720001 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References