Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4806159..4806969 Replicon chromosome
Accession NZ_CP092793
Organism Klebsiella pneumoniae strain XH1507

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag ML258_RS23860 Protein ID WP_002887280.1
Coordinates 4806159..4806692 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ML258_RS23865 Protein ID WP_002887278.1
Coordinates 4806703..4806969 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ML258_RS23855 (ML258_23855) 4804990..4806111 + 1122 WP_002887282.1 cupin domain-containing protein -
ML258_RS23860 (ML258_23860) 4806159..4806692 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
ML258_RS23865 (ML258_23865) 4806703..4806969 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
ML258_RS23870 (ML258_23870) 4807072..4808505 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
ML258_RS23875 (ML258_23875) 4808495..4809178 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
ML258_RS23880 (ML258_23880) 4809350..4810735 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
ML258_RS23885 (ML258_23885) 4810753..4811097 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T237186 WP_002887280.1 NZ_CP092793:c4806692-4806159 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T237186 NZ_LR782232:2963454-2963561 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT237186 WP_002887278.1 NZ_CP092793:c4806969-4806703 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT237186 NZ_LR782232:c2963407-2963341 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References