Detailed information of TA system TA237171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2069..2543
• Replicon: plasmid pXH1508-5
• Accession: NZ_CP092791
• Organism: Klebsiella pneumoniae strain XH1508

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: ML259_RS29745
• Protein ID: WP_004197762.1
• Coordinates: 2069..2335 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: ML259_RS29750
• Protein ID: WP_000879771.1
• Coordinates: 2319..2543 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ML259_RS29730 (ML259_29730)	686..1012	+	327	WP_050516389.1	hypothetical protein	-
ML259_RS29735 (ML259_29735)	1038..1583	+	546	WP_023317533.1	hypothetical protein	-
ML259_RS29740 (ML259_29740)	1649..1999	+	351	WP_004197756.1	hypothetical protein	-
ML259_RS29745 (ML259_29745)	2069..2335	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ML259_RS29750 (ML259_29750)	2319..2543	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
ML259_RS29755 (ML259_29755)	3210..3422	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
ML259_RS29760 (ML259_29760)	3429..3938	-	510	WP_032433972.1	MbeB family mobilization protein	-
ML259_RS29765 (ML259_29765)	3973..4407	-	435	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
ML259_RS29770 (ML259_29770)	4616..4960	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
ML259_RS29775 (ML259_29775)	4950..5126	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T237171 WP_004197762.1 NZ_CP092791:c2335-2069 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T237171 NZ_LR778301:c2905506-2905252 [Denitratisoma oestradiolicum]
GTGAAGCTGATTTTTTCTGACGAGGCATGGGAAGACTACCTGTATTGGCAGAAGCAAGACAAGCGCATGGTGGAACGAAT
CAACAAGCTGATTCGGGAAACGGAGCGCGAGCCATTTAACGGCGTAGGCAAGCCGGAGCCGTTGAAGCACGCGCTATCGG
GATTTTGGTCTCGGCGGATCACAGATGAACATCGCATGGTGTATCGCGTCGTGAACGATGCGCTGGAAATTGTGCAACTC
CGTTTCCACTACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT237171 WP_000879771.1 NZ_CP092791:c2543-2319 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT237171 NZ_LR778301:c2905754-2905503 [Denitratisoma oestradiolicum]
ATGGATGCGATTACCTACACTACAGCTCGCGCGAACTTGGCGACTACCATGGATCGGGTGTGCAACGACCACGAGGCGCT
GATAATTACCCGCAACGGCGAACAGGCCGTTGTGATGCTTTCGCTCGAAGATTTCAAGGCGCTTGAAGAAACGGCCTACC
TTCTGCGCACACCGGCCAACGCCAAGCGCCTGCTCTCGGCGATTGCTCAGTTGAACGCTGGGAAAGGTGTTGAGCGGAAG
CTGGCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5