Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 27377..27959 Replicon plasmid pXH1508-4
Accession NZ_CP092790
Organism Klebsiella pneumoniae strain XH1508

Toxin (Protein)


Gene name PumA Uniprot ID B1VJ82
Locus tag ML259_RS29680 Protein ID WP_012368903.1
Coordinates 27377..27673 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID B1VJ81
Locus tag ML259_RS29685 Protein ID WP_012368902.1
Coordinates 27675..27959 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ML259_RS29645 (ML259_29645) 22476..23453 - 978 WP_212656691.1 replication initiator protein A -
ML259_RS29650 (ML259_29650) 24435..24959 + 525 WP_212656692.1 J domain-containing protein -
ML259_RS29655 (ML259_29655) 25213..25764 + 552 WP_212656693.1 hypothetical protein -
ML259_RS29660 (ML259_29660) 25786..26052 + 267 WP_212656694.1 hypothetical protein -
ML259_RS29665 (ML259_29665) 26042..26272 + 231 WP_073546961.1 hypothetical protein -
ML259_RS29670 (ML259_29670) 26645..26857 + 213 WP_073546991.1 hypothetical protein -
ML259_RS29675 (ML259_29675) 26904..27047 + 144 WP_212656695.1 hypothetical protein -
ML259_RS29870 27123..27257 + 135 WP_259493503.1 hypothetical protein -
ML259_RS29680 (ML259_29680) 27377..27673 + 297 WP_012368903.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ML259_RS29685 (ML259_29685) 27675..27959 + 285 WP_012368902.1 putative addiction module antidote protein Antitoxin
ML259_RS29690 (ML259_29690) 28667..29215 + 549 WP_212656696.1 TraY domain protein -
ML259_RS29695 (ML259_29695) 29223..30383 + 1161 WP_212656697.1 MobP1 family relaxase -
ML259_RS29700 (ML259_29700) 30393..30749 + 357 WP_212656698.1 DNA distortion polypeptide 3 -
ML259_RS29705 (ML259_29705) 30726..31250 - 525 WP_212656699.1 restriction endonuclease -
ML259_RS29710 (ML259_29710) 31342..31671 - 330 WP_113355598.1 hypothetical protein -
ML259_RS29715 (ML259_29715) 31671..31916 - 246 WP_113355596.1 hypothetical protein -
ML259_RS29720 (ML259_29720) 32216..32497 - 282 WP_113355595.1 CopG family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..34012 34012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Uncharacterized protein HI1420

(3-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11325.21 Da        Isoelectric Point: 10.2910

>T237170 WP_012368903.1 NZ_CP092790:27377-27673 [Klebsiella pneumoniae]
MIEIRQTEEVKKWLKGLKDKIAKAKILTRIERMKEGNYGDVEPIGDGYSELRIHQGKGYRVYFANRNNEIILLLCGGDKT
TQQADIKKAKQIAKKWGF

Download         Length: 297 bp

>T237170 NZ_LR778301:c75284-74007 [Denitratisoma oestradiolicum]
ATGAGCCGGGTCCTGGAAGTCTGGCTGCTGGGGCAACACGTCGGACAACTGGCTCAAGTCGATGGCCGGCTGAACTTCAG
CTACTCACCGCAATGGCTGCAATCCGCAAACGCACGTCCACTGTCACACCACCTCCCCTTACAGGCAGGGTCTTTTGACG
ACAAGGCCACGCGCCCCTTCTTTGCCGGCCTGCTGCCAGAGGGCGACAAGCGCAGGCTGATCGCCCAAACGCTACACGTC
TCGCGCCATAATGATTTTGCACTGCTGGACGGGATTGGTGGCGAATGTGCTGGCGCAGTCAGCCTGCTGGAAACTGGCCA
ACAACCCGACGCCATCCAGTCCGCGCCAGTGGTGCGCTGGCTGGACCAGTCAGAACTGGTCGCGATCCTGGATGAGCTGC
CCCGCCGCCCCATGTTGGCAGGCGACCAGGGGCTGCGTCTGTCCTTGGCCGGCGCTCAGGACAAGCTGCCGGTGACCTTC
GCTGATGGACGCATGGGTTTGCCTCTTCAGAACACCCCAAGCTCTCACATCCTCAAGCCGGCAATCTCGAGAGTTGAGGG
CAGCGTATTCAACGAAGGGTTCTGCCTGGCCCTGGCGGCACAGATAAAGCTGTCTGTTGCCAGGGCAGCCATTCACCGGG
TGGCTGACCGCGAGTATTTGCTGGTGGAACGCTACGACCGTGTGCGCCAACCCGACGGTGCCTTGCAGCGGCTGCATCAG
GAAGACTTCTGCCAGGCACTGGGCGTCGCACCGGAATACAAGTACCAAAACGAGGGCGGTCCAGATCTGGCCCAATGCTT
CGGGCTGGTGCGCAAAGCAACACGCCCCAGCGCCCCGCACATTTTGCGCTTGCTGGACTACGCGATTTTCAACGCCATGA
TCGGCAACCACGATGCACACGCCAAGAACTTCTCCCTGATCTACACCGAGCGCGGCGCGGCACTGGCACCACTGTACGAT
ACCTTGTCGACAGCGGTGTACGCCAATCTCACGGACAAGATGGCGATGAAGATCGGCAGCAAGTACAAGTTCACCGAACT
CCAGTCGCGGCATTGGGAACAGTTTGCGCAAGCGGCAGGACTGTCTCCAGCGCAGGTCAAAAAGCGGGTTCTGGCCATCG
CACGCCAGTTACCCCACGAAGCCGCCGCCCTGCGTTCATCGTTCGTCACCCAGGGCCTGGATCACGACATACTCGGCAAG
ATCGTTAATCTCATCGAGCGGCGTTGCCTGCTGACCAGCAAGCGGCTTGAAACCGCAGGACCTGGCGACGATGAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10302.61 Da        Isoelectric Point: 4.6286

>AT237170 WP_012368902.1 NZ_CP092790:27675-27959 [Klebsiella pneumoniae]
MKTKPELFDVVDYLEDEEDIQAYLNAALDEDDESYFLAALGNIARARNISQLSKETGMSREGIYKALSGTGNPSFKTISK
ISKALGLKIHFAGA

Download         Length: 285 bp

>AT237170 NZ_LR778301:c75526-75281 [Denitratisoma oestradiolicum]
ATGCCTACATTGATTGAAACTCCACAACATCTGGGCGACGCAGTGCGACGCGCCCGCAAAGCGCTGGGACTGACCCAACC
CCAATTGGCGCTCGTAGCAGGCGTTGGCGTGCGATTCATTGTTGACCTGGAAGCAGGCAAACCCACCGTCCGGCTGGAGA
ACGTCCTGCGTGTCCTACAGGCGCTGGGAGCAACGTTGAGCTTGGCAGGGCTGGAGGCAAGTCAGACGCAGGAGGCCCAA
AAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I3XCB7

Antitoxin

Source ID Structure
AlphaFold DB A0A1I3XCJ1

References