Detailed information of TA system TA237164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 124164..124834
• Replicon: plasmid pXH1508-1
• Accession: NZ_CP092787
• Organism: Klebsiella pneumoniae strain XH1508

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: ML259_RS27760
• Protein ID: WP_004213072.1
• Coordinates: 124164..124607 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: ML259_RS27765
• Protein ID: WP_004213073.1
• Coordinates: 124604..124834 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ML259_RS27725 (ML259_27725)	119575..119850	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
ML259_RS27730 (ML259_27730)	119913..120404	+	492	WP_011251327.1	DM13 domain-containing protein	-
ML259_RS27735 (ML259_27735)	120453..121373	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
ML259_RS27740 (ML259_27740)	121464..121867	+	404	Protein_140	GAF domain-containing protein	-
ML259_RS27745 (ML259_27745)	122385..123020	-	636	Protein_141	mucoid phenotype regulator RmpA2	-
ML259_RS27750 (ML259_27750)	123437..123741	+	305	Protein_142	transposase	-
ML259_RS27755 (ML259_27755)	123764..124015	-	252	WP_186987481.1	hypothetical protein	-
ML259_RS27760 (ML259_27760)	124164..124607	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
ML259_RS27765 (ML259_27765)	124604..124834	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
ML259_RS27770 (ML259_27770)	125442..126575	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
ML259_RS27775 (ML259_27775)	126591..126884	+	294	WP_004213076.1	hypothetical protein	-
ML259_RS27780 (ML259_27780)	126874..127080	-	207	WP_004213077.1	hypothetical protein	-
ML259_RS27785 (ML259_27785)	127432..127722	+	291	WP_004213078.1	hypothetical protein	-
ML259_RS27790 (ML259_27790)	127712..128611	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..198615	198615

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T237164 WP_004213072.1 NZ_CP092787:c124607-124164 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T237164 NZ_LR778153:4012657-4012935 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT237164 WP_004213073.1 NZ_CP092787:c124834-124604 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT237164 NZ_LR778153:4012394-4012654 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620