Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5391792..5392417 Replicon chromosome
Accession NZ_CP092786
Organism Klebsiella pneumoniae strain XH1508

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ML259_RS26675 Protein ID WP_002882817.1
Coordinates 5391792..5392175 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ML259_RS26680 Protein ID WP_004150355.1
Coordinates 5392175..5392417 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ML259_RS26660 (ML259_26660) 5389158..5390060 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ML259_RS26665 (ML259_26665) 5390057..5390692 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ML259_RS26670 (ML259_26670) 5390689..5391618 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ML259_RS26675 (ML259_26675) 5391792..5392175 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ML259_RS26680 (ML259_26680) 5392175..5392417 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
ML259_RS26685 (ML259_26685) 5392622..5393539 + 918 WP_002882812.1 alpha/beta hydrolase -
ML259_RS26690 (ML259_26690) 5393553..5394494 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ML259_RS26695 (ML259_26695) 5394539..5394976 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
ML259_RS26700 (ML259_26700) 5394973..5395833 - 861 WP_002882807.1 virulence factor BrkB family protein -
ML259_RS26705 (ML259_26705) 5395827..5396426 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T237160 WP_002882817.1 NZ_CP092786:c5392175-5391792 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T237160 NZ_LR778153:2961700-2961807 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT237160 WP_004150355.1 NZ_CP092786:c5392417-5392175 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT237160 NZ_LR778153:c2961653-2961587 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References