Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4807064..4807874 Replicon chromosome
Accession NZ_CP092786
Organism Klebsiella pneumoniae strain XH1508

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag ML259_RS23875 Protein ID WP_002887280.1
Coordinates 4807064..4807597 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ML259_RS23880 Protein ID WP_002887278.1
Coordinates 4807608..4807874 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ML259_RS23870 (ML259_23870) 4805895..4807016 + 1122 WP_002887282.1 cupin domain-containing protein -
ML259_RS23875 (ML259_23875) 4807064..4807597 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
ML259_RS23880 (ML259_23880) 4807608..4807874 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
ML259_RS23885 (ML259_23885) 4807977..4809410 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
ML259_RS23890 (ML259_23890) 4809400..4810083 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
ML259_RS23895 (ML259_23895) 4810255..4811640 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
ML259_RS23900 (ML259_23900) 4811658..4812002 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T237157 WP_002887280.1 NZ_CP092786:c4807597-4807064 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T237157 NZ_LR778153:2300460-2300563 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT237157 WP_002887278.1 NZ_CP092786:c4807874-4807608 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT237157 NZ_LR778153:c2300601-2300330 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References