Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4193951..4194570 Replicon chromosome
Accession NZ_CP092786
Organism Klebsiella pneumoniae strain XH1508

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ML259_RS20930 Protein ID WP_002892050.1
Coordinates 4194352..4194570 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag ML259_RS20925 Protein ID WP_002892066.1
Coordinates 4193951..4194325 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ML259_RS20915 (ML259_20915) 4189103..4190296 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ML259_RS20920 (ML259_20920) 4190319..4193465 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
ML259_RS20925 (ML259_20925) 4193951..4194325 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
ML259_RS20930 (ML259_20930) 4194352..4194570 + 219 WP_002892050.1 HHA domain-containing protein Toxin
ML259_RS20935 (ML259_20935) 4194729..4195295 + 567 WP_002892030.1 maltose O-acetyltransferase -
ML259_RS20940 (ML259_20940) 4195267..4195407 - 141 WP_004147370.1 hypothetical protein -
ML259_RS20945 (ML259_20945) 4195428..4195898 + 471 WP_002892026.1 YlaC family protein -
ML259_RS20950 (ML259_20950) 4195873..4197324 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
ML259_RS20955 (ML259_20955) 4197425..4198123 + 699 WP_002892021.1 GNAT family protein -
ML259_RS20960 (ML259_20960) 4198120..4198260 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ML259_RS20965 (ML259_20965) 4198260..4198523 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T237156 WP_002892050.1 NZ_CP092786:4194352-4194570 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T237156 NZ_LR778153:1582508-1582906 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT237156 WP_002892066.1 NZ_CP092786:4193951-4194325 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT237156 NZ_LR778153:1582281-1582508 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References