Detailed information of TA system TA237142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 74658..75229
• Replicon: plasmid unnamed1
• Accession: NZ_CP092785
• Organism: Enterococcus sp. LX10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R3HR30
• Locus tag: MKZ30_RS13215
• Protein ID: WP_001278770.1
• Coordinates: 74658..74999 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MKZ30_RS13220
• Protein ID: WP_249958820.1
• Coordinates: 74999..75229 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKZ30_RS13185 (70519)	70519..70794	-	276	Protein_69	type II toxin-antitoxin system YafQ family toxin	-
MKZ30_RS13190 (70787)	70787..71053	-	267	WP_016614869.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
MKZ30_RS13195 (71181)	71181..71291	-	111	Protein_71	ATP-binding protein	-
MKZ30_RS13200 (71752)	71752..72549	-	798	WP_202473241.1	GH25 family lysozyme	-
MKZ30_RS13205 (72759)	72759..73361	-	603	WP_010821245.1	Fic family protein	-
MKZ30_RS13210 (73608)	73608..74546	-	939	WP_010829201.1	hypothetical protein	-
MKZ30_RS13215 (74658)	74658..74999	-	342	WP_001278770.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKZ30_RS13220 (74999)	74999..75229	-	231	WP_249958820.1	AbrB family transcriptional regulator	Antitoxin
MKZ30_RS13225 (75433)	75433..76053	+	621	WP_249958821.1	recombinase family protein	-
MKZ30_RS13230 (76043)	76043..76357	+	315	WP_016616017.1	hypothetical protein	-
MKZ30_RS13235 (76351)	76351..76575	+	225	WP_086318235.1	hypothetical protein	-
MKZ30_RS13240 (76637)	76637..76846	+	210	WP_002363054.1	hypothetical protein	-
MKZ30_RS13245 (77187)	77187..77438	+	252	WP_010711381.1	hypothetical protein	-
MKZ30_RS13250 (77492)	77492..77929	-	438	WP_208784554.1	hypothetical protein	-
MKZ30_RS13255 (78021)	78021..78272	+	252	WP_086318238.1	hypothetical protein	-
MKZ30_RS13260 (78388)	78388..79704	+	1317	WP_249958822.1	Y-family DNA polymerase	-
MKZ30_RS13265 (79708)	79708..80214	+	507	WP_202254788.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82739	82739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13220.44 Da
• Isoelectric Point: 8.8595

>T237142 WP_001278770.1 NZ_CP092785:c74999-74658 [Enterococcus sp. LX10]
MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T237142 NZ_LR778152:3800729-3801106 [Escherichia coli]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAGTTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTACGAGTATGAAGGCGTTTCCGATCTTT
GGCTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATTTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACAGCCTCATACTTGCTGCAAACCCAGAGTTTGTGGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACACGGAACCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8536.50 Da
• Isoelectric Point: 4.3855

>AT237142 WP_249958820.1 NZ_CP092785:c75229-74999 [Enterococcus sp. LX10]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGLEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT237142 NZ_LR778152:3800511-3800732 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGACAGAACCTTGCATTGGTTCTGGAAACAGCAGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCCGCTGTGATTATTAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CGGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: R3HR30