Detailed information of TA system TA237129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1272473..1273390
• Replicon: chromosome
• Accession: NZ_CP092778
• Organism: Bacillus amyloliquefaciens strain WF2020

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: MK616_RS06675
• Protein ID: WP_007407256.1
• Coordinates: 1272644..1273390 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: MK616_RS06670
• Protein ID: WP_003154807.1
• Coordinates: 1272473..1272643 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MK616_RS06625 (MK616_06625)	1267711..1269333	+	1623	WP_240680199.1	pyocin knob domain-containing protein	-
MK616_RS06630 (MK616_06630)	1269346..1269498	+	153	WP_240680200.1	hypothetical protein	-
MK616_RS06635 (MK616_06635)	1269479..1269712	+	234	WP_240680201.1	XkdW family protein	-
MK616_RS06640 (MK616_06640)	1269717..1269914	+	198	WP_007610833.1	XkdX family protein	-
MK616_RS06645 (MK616_06645)	1269971..1270732	+	762	WP_100261724.1	phage portal protein	-
MK616_RS06650 (MK616_06650)	1270784..1271047	+	264	WP_100261725.1	hemolysin XhlA family protein	-
MK616_RS06655 (MK616_06655)	1271061..1271324	+	264	WP_003154813.1	phage holin	-
MK616_RS06660 (MK616_06660)	1271338..1272216	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
MK616_RS06665 (MK616_06665)	1272251..1272376	-	126	WP_003154809.1	hypothetical protein	-
MK616_RS06670 (MK616_06670)	1272473..1272643	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
MK616_RS06675 (MK616_06675)	1272644..1273390	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MK616_RS06680 (MK616_06680)	1273495..1274493	-	999	WP_003154805.1	inorganic phosphate transporter	-
MK616_RS06685 (MK616_06685)	1274506..1275123	-	618	WP_003154804.1	DUF47 domain-containing protein	-
MK616_RS06690 (MK616_06690)	1275409..1276725	-	1317	WP_163093916.1	amino acid permease	-
MK616_RS06695 (MK616_06695)	1277047..1277997	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T237129 WP_007407256.1 NZ_CP092778:c1273390-1272644 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T237129 NZ_LR778152:846644-846746 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT237129 WP_003154807.1 NZ_CP092778:c1272643-1272473 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT237129 NZ_LR778152:c846784-846516 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14