Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 476555..477192 | Replicon | chromosome |
| Accession | NZ_CP092778 | ||
| Organism | Bacillus amyloliquefaciens strain WF2020 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | MK616_RS02415 | Protein ID | WP_003156187.1 |
| Coordinates | 476842..477192 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | MK616_RS02410 | Protein ID | WP_003156188.1 |
| Coordinates | 476555..476836 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MK616_RS02390 (MK616_02390) | 472920..473519 | - | 600 | WP_003156193.1 | rhomboid family intramembrane serine protease | - |
| MK616_RS02395 (MK616_02395) | 473612..473977 | + | 366 | WP_014304402.1 | holo-ACP synthase | - |
| MK616_RS02400 (MK616_02400) | 474142..475149 | + | 1008 | WP_007410230.1 | outer membrane lipoprotein carrier protein LolA | - |
| MK616_RS02405 (MK616_02405) | 475266..476435 | + | 1170 | WP_021495079.1 | alanine racemase | - |
| MK616_RS02410 (MK616_02410) | 476555..476836 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| MK616_RS02415 (MK616_02415) | 476842..477192 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| MK616_RS02420 (MK616_02420) | 477310..478131 | + | 822 | WP_003156182.1 | STAS domain-containing protein | - |
| MK616_RS02425 (MK616_02425) | 478136..478501 | + | 366 | WP_007609584.1 | RsbT antagonist protein RsbS | - |
| MK616_RS02430 (MK616_02430) | 478504..478905 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| MK616_RS02435 (MK616_02435) | 478917..479924 | + | 1008 | WP_007609589.1 | PP2C family protein-serine/threonine phosphatase | - |
| MK616_RS02440 (MK616_02440) | 479988..480317 | + | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| MK616_RS02445 (MK616_02445) | 480314..480796 | + | 483 | WP_007609591.1 | anti-sigma B factor RsbW | - |
| MK616_RS02450 (MK616_02450) | 480762..481550 | + | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| MK616_RS02455 (MK616_02455) | 481550..482152 | + | 603 | WP_007410234.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T237128 WP_003156187.1 NZ_CP092778:476842-477192 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T237128 NZ_LR778152:630262-630516 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAGCCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAGCCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT237128 WP_003156188.1 NZ_CP092778:476555-476836 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT237128 NZ_LR778152:630014-630265 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|