Detailed information of TA system TA023712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 374988..375516
• Replicon: chromosome II
• Accession: NC_012580
• Organism: Vibrio cholerae M66-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: VCM66_RS15180
• Protein ID: WP_000221352.1
• Coordinates: 374988..375278 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: VCM66_RS15185
• Protein ID: WP_000213183.1
• Coordinates: 375268..375516 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCM66_RS15155	371229..372236	+	1008	WP_000093800.1	DUF4365 domain-containing protein	-
VCM66_RS19385	372233..372343	+	111	Protein_363	DUF3265 domain-containing protein	-
VCM66_RS18900	372527..372707	+	181	Protein_364	DUF645 family protein	-
VCM66_RS15160	372974..373261	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCM66_RS15165	373272..373589	+	318	WP_001232701.1	HigA family addiction module antidote protein	-
VCM66_RS19565	373586..373696	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
VCM66_RS15170	373724..374200	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
VCM66_RS15175	374337..374852	+	516	WP_001201532.1	lipocalin family protein	-
VCM66_RS15180	374988..375278	-	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VCM66_RS15185	375268..375516	-	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
VCM66_RS15200	376087..376740	+	654	WP_000036957.1	site-specific DNA-methyltransferase	-
VCM66_RS15205	377012..377437	+	426	WP_000415748.1	hypothetical protein	-
VCM66_RS15210	377645..378040	+	396	WP_000649527.1	DUF3465 domain-containing protein	-
VCM66_RS15215	378191..378721	+	531	WP_000415563.1	hypothetical protein	-
VCM66_RS15220	378859..379359	+	501	WP_000789723.1	hypothetical protein	-
VCM66_RS15225	379515..379913	+	399	WP_001882293.1	hypothetical protein	-
VCM66_RS15230	380013..380477	-	465	WP_000140244.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	305869..409778	103909
	-	inside	Integron	-	-	310949..409402	98453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T23712 WP_000221352.1 NC_012580:c375278-374988 [Vibrio cholerae M66-2]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T23712 NC_012580:c375278-374988 [Vibrio cholerae M66-2]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT23712 WP_000213183.1 NC_012580:c375516-375268 [Vibrio cholerae M66-2]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT23712 NC_012580:c375516-375268 [Vibrio cholerae M66-2]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8