Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4058722..4059341 Replicon chromosome
Accession NZ_CP092765
Organism Klebsiella pneumoniae strain bat15

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MKZ43_RS20230 Protein ID WP_002892050.1
Coordinates 4059123..4059341 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MKZ43_RS20225 Protein ID WP_002892066.1
Coordinates 4058722..4059096 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKZ43_RS20215 (MKZ43_20215) 4053874..4055067 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MKZ43_RS20220 (MKZ43_20220) 4055090..4058236 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
MKZ43_RS20225 (MKZ43_20225) 4058722..4059096 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MKZ43_RS20230 (MKZ43_20230) 4059123..4059341 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MKZ43_RS20235 (MKZ43_20235) 4059500..4060066 + 567 WP_002892030.1 maltose O-acetyltransferase -
MKZ43_RS20240 (MKZ43_20240) 4060038..4060178 - 141 WP_004147370.1 hypothetical protein -
MKZ43_RS20245 (MKZ43_20245) 4060199..4060669 + 471 WP_002892026.1 YlaC family protein -
MKZ43_RS20250 (MKZ43_20250) 4060644..4062095 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MKZ43_RS20255 (MKZ43_20255) 4062196..4062894 + 699 WP_002892021.1 GNAT family protein -
MKZ43_RS20260 (MKZ43_20260) 4062891..4063031 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MKZ43_RS20265 (MKZ43_20265) 4063031..4063294 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T237119 WP_002892050.1 NZ_CP092765:4059123-4059341 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T237119 NZ_LR778151:c2714953-2714618 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT237119 WP_002892066.1 NZ_CP092765:4058722-4059096 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT237119 NZ_LR778151:c2715201-2714953 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References