Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-higA/GNAT-HigA |
| Location | 373272..374200 | Replicon | chromosome II |
| Accession | NC_012580 | ||
| Organism | Vibrio cholerae M66-2 | ||
Toxin (Protein)
| Gene name | phd | Uniprot ID | Q9KMC2 |
| Locus tag | VCM66_RS15170 | Protein ID | WP_001047180.1 |
| Coordinates | 373724..374200 (+) | Length | 159 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | VCM66_RS15165 | Protein ID | WP_001232701.1 |
| Coordinates | 373272..373589 (+) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| VCM66_RS15140 | 369181..369630 | + | 450 | WP_001894449.1 | hypothetical protein | - |
| VCM66_RS15155 | 371229..372236 | + | 1008 | WP_000093800.1 | DUF4365 domain-containing protein | - |
| VCM66_RS19385 | 372233..372343 | + | 111 | Protein_363 | DUF3265 domain-containing protein | - |
| VCM66_RS18900 | 372527..372707 | + | 181 | Protein_364 | DUF645 family protein | - |
| VCM66_RS15160 | 372974..373261 | + | 288 | WP_001162670.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| VCM66_RS15165 | 373272..373589 | + | 318 | WP_001232701.1 | HigA family addiction module antidote protein | Antitoxin |
| VCM66_RS19565 | 373586..373696 | + | 111 | WP_134814058.1 | DUF3265 domain-containing protein | - |
| VCM66_RS15170 | 373724..374200 | + | 477 | WP_001047180.1 | GNAT family N-acetyltransferase | Toxin |
| VCM66_RS15175 | 374337..374852 | + | 516 | WP_001201532.1 | lipocalin family protein | - |
| VCM66_RS15180 | 374988..375278 | - | 291 | WP_000221352.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| VCM66_RS15185 | 375268..375516 | - | 249 | WP_000213183.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| VCM66_RS15200 | 376087..376740 | + | 654 | WP_000036957.1 | site-specific DNA-methyltransferase | - |
| VCM66_RS15205 | 377012..377437 | + | 426 | WP_000415748.1 | hypothetical protein | - |
| VCM66_RS15210 | 377645..378040 | + | 396 | WP_000649527.1 | DUF3465 domain-containing protein | - |
| VCM66_RS15215 | 378191..378721 | + | 531 | WP_000415563.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 305869..409778 | 103909 | |
| - | inside | Integron | - | - | 310949..409402 | 98453 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 159 a.a. Molecular weight: 18366.18 Da Isoelectric Point: 6.7088
>T23711 WP_001047180.1 NC_012580:373724-374200 [Vibrio cholerae M66-2]
MNLEEFQESDFDLLIKWIDSDELNYLWGGPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVSTEIGTRAFNGKLWDLVRMEKRL
MNLEEFQESDFDLLIKWIDSDELNYLWGGPAYVFPLTYEQIHSHCSKAEVFPYLLKVKGRHAGFVELYKVTDEQYRICRV
FISNAYRGQGLSKSMLMLLIDKARLDFSATKLSLGVFEQNTVARKCYESLGFEVVSTEIGTRAFNGKLWDLVRMEKRL
Download Length: 477 bp
>T23711 NC_012580:373724-374200 [Vibrio cholerae M66-2]
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTGGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTTCGACAGAAATTGGTACAAGAGCTTTTAATGGTAAGTTATGGGATCTGGTGCGAATGGAAAAACGGTTATAA
ATGAACTTAGAAGAGTTTCAAGAATCGGATTTTGACCTGCTTATAAAATGGATTGACTCAGATGAGTTGAACTATTTATG
GGGTGGCCCTGCGTATGTTTTCCCGCTGACTTATGAACAGATCCATTCCCACTGTAGCAAAGCGGAAGTATTTCCCTATC
TGCTAAAAGTGAAAGGTAGACATGCCGGGTTTGTTGAGTTGTACAAAGTCACCGATGAGCAATATCGGATTTGCCGTGTC
TTTATTTCAAATGCTTATCGTGGGCAAGGGCTATCAAAATCGATGTTGATGTTATTAATCGATAAAGCCCGATTAGATTT
TTCTGCAACCAAGCTTAGCTTAGGCGTTTTTGAACAGAACACAGTTGCTAGAAAATGCTATGAGTCATTAGGTTTTGAAG
TGGTTTCGACAGAAATTGGTACAAGAGCTTTTAATGGTAAGTTATGGGATCTGGTGCGAATGGAAAAACGGTTATAA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11705.63 Da Isoelectric Point: 10.4711
>AT23711 WP_001232701.1 NC_012580:373272-373589 [Vibrio cholerae M66-2]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART
Download Length: 318 bp
>AT23711 NC_012580:373272-373589 [Vibrio cholerae M66-2]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|