Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 17449..18092 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP092761 | ||
| Organism | Klebsiella pneumoniae strain bat16 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | MKZ45_RS27640 | Protein ID | WP_001044770.1 |
| Coordinates | 17676..18092 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | MKZ45_RS27635 | Protein ID | WP_001261282.1 |
| Coordinates | 17449..17679 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MKZ45_RS27595 (MKZ45_27585) | 12647..13120 | + | 474 | WP_004152341.1 | YkgJ family cysteine cluster protein | - |
| MKZ45_RS27600 (MKZ45_27590) | 13212..13442 | + | 231 | WP_011977773.1 | hypothetical protein | - |
| MKZ45_RS27605 (MKZ45_27595) | 14334..15116 | - | 783 | WP_004152340.1 | site-specific integrase | - |
| MKZ45_RS27610 (MKZ45_27600) | 15116..15448 | - | 333 | WP_004152339.1 | hypothetical protein | - |
| MKZ45_RS27615 (MKZ45_27605) | 15455..15853 | - | 399 | WP_004171440.1 | hypothetical protein | - |
| MKZ45_RS27620 (MKZ45_27610) | 15879..16208 | - | 330 | WP_004152337.1 | hypothetical protein | - |
| MKZ45_RS27625 (MKZ45_27615) | 16236..16544 | - | 309 | WP_004152336.1 | hypothetical protein | - |
| MKZ45_RS27630 (MKZ45_27620) | 17031..17492 | - | 462 | WP_072093212.1 | hypothetical protein | - |
| MKZ45_RS27635 (MKZ45_27625) | 17449..17679 | + | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| MKZ45_RS27640 (MKZ45_27630) | 17676..18092 | + | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| MKZ45_RS27645 (MKZ45_27635) | 18166..18855 | + | 690 | Protein_19 | AAA family ATPase | - |
| MKZ45_RS27650 (MKZ45_27640) | 18910..19607 | + | 698 | WP_223174630.1 | IS1 family transposase | - |
| MKZ45_RS27655 (MKZ45_27645) | 19623..19733 | + | 111 | Protein_21 | mercuric transport protein periplasmic component | - |
| MKZ45_RS27660 (MKZ45_27650) | 19769..20191 | + | 423 | WP_001340589.1 | organomercurial transporter MerC | - |
| MKZ45_RS27665 (MKZ45_27655) | 20243..21937 | + | 1695 | WP_000105636.1 | mercury(II) reductase | - |
| MKZ45_RS27670 (MKZ45_27660) | 21955..22317 | + | 363 | WP_001277456.1 | mercury resistance co-regulator MerD | - |
| MKZ45_RS27675 (MKZ45_27665) | 22314..22550 | + | 237 | WP_000993386.1 | broad-spectrum mercury transporter MerE | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaTEM-1A / blaOXA-9 / blaKPC-3 | - | 1..118949 | 118949 | |
| - | flank | IS/Tn | - | - | 11259..12527 | 1268 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T237105 WP_001044770.1 NZ_CP092761:17676-18092 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T237105 NZ_LR778150:c4604360-4604049 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT237105 WP_001261282.1 NZ_CP092761:17449-17679 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT237105 NZ_LR778150:c4604048-4603758 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |